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首页> 外文期刊>Journal of biochemical and molecular toxicology >Scoparia dulcis, a traditional antidiabetic plant, protects against streptozotocin induced oxidative stress and apoptosis in vitro and in vivo.
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Scoparia dulcis, a traditional antidiabetic plant, protects against streptozotocin induced oxidative stress and apoptosis in vitro and in vivo.

机译：Scoparia dulcis是一种传统的抗糖尿病植物，在体外和体内均可抵抗链脲佐菌素诱导的氧化应激和细胞凋亡。

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Oxidative stress is implicated in the pathogenesis of diabetic complications. The experiments were performed on normal and experimental male Wistar rats treated with Scoparia dulcis plant extract (SPEt). The effect of SPEt was tested on streptozotocin (STZ) treated Rat insulinoma cell lines (RINm5F cells) and isolated islets in vitro. Administration of an aqueous extract of Scoparia dulcis by intragastric intubation (po) at a dose of 200 mg/kg body weight significantly decreased the blood glucose and lipid peroxidative marker thiobarbituric acid reactive substances (TBARS) with significant increase in the activities of plasma insulin, pancreatic superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) in streptozotocin diabetic rats at the end of 15 days treatment. Streptozotocin at a dose of 10 mug/mL evoked 6-fold stimulation of insulin secretion from isolated islets indicating its insulin secretagogue activity. The extract markedly reduced the STZ-induced lipidperoxidation in RINm5Fcells. Further, SPEt protected STZ-mediated cytotoxicity and nitric oxide (NO) production in RINm5F cells. Treatment of RINm5F cells with 5 mM STZ and 10 mug of SPEt completely abrogated apoptosis induced by STZ, suggesting the involvement of oxidative stress. Flow cytometric assessment on the level of intracellular peroxides using fluorescent probe 2'7'-dichlorofluorescein diacetate (DCF-DA) confirmed that STZ (46%) induced an intracellular oxidative stress in RINm5F cells, which was suppressed by SPEt (21%). In addition, SPEt also reduced (33%) the STZ-induced apoptosis (72%) in RINm5F cells indicating the mode of protection of SPEt on RIN m5Fcells, islets, and pancreatic beta-cell mass (histopathological observations). Present study thus confirms antihyperglycemic effect of SPEt and also demonstrated the consistently strong antioxidant properties of Scoparia dulcis used in the traditional medicine. (c) 2004 Wiley Periodicals, Inc. J Biochem Mol Toxicol 18:261-272, 2004 Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20035.
机译：氧化应激与糖尿病并发症的发病机理有关。实验是在正常和实验雄性Wistar大鼠身上进行的，该大鼠用Scoparia dulcis植物提取物（SPEt）处理。测试了SPEt对链脲佐菌素（STZ）处理的大鼠胰岛素瘤细胞系（RINm5F细胞）和离体胰岛的影响。以200 mg / kg体重的剂量通过胃内插管（po）施用Scoparia dulcis的水提取物，可显着降低血糖和脂质过氧化标志物硫代巴比妥酸反应性物质（TBARS），并显着增加血浆胰岛素的活性，治疗结束15天后，链脲佐菌素糖尿病大鼠的胰腺超氧化物歧化酶（SOD），过氧化氢酶（CAT）和还原型谷胱甘肽（GSH）降低。剂量为10杯/毫升的链脲佐菌素引起从孤立的胰岛中胰岛素分泌的6倍刺激，表明其胰岛素促分泌活性。该提取物显着减少了RINm5F细胞中STZ诱导的脂质过氧化。此外，SPEt保护RINm5F细胞中STZ介导的细胞毒性和一氧化氮（NO）的产生。用5 mM STZ和10杯SPEt处理RINm5F细胞可完全消除STZ诱导的细胞凋亡，提示其参与了氧化应激。使用荧光探针2'7'-dichlorofluorescein diacetate（DCF-DA）对细胞内过氧化物水平进行流式细胞术评估，证实STZ（46％）诱导RINm5F细胞内的细胞内氧化应激，被SPEt（21％）抑制。此外，SPEt还减少了STm诱导的RINm5F细胞凋亡（33％）（72％），表明SPEt对RIN m5F细胞，胰岛和胰岛β细胞团的保护方式（组织病理学观察）。因此，目前的研究证实了SPEt的降血糖作用，并证明了传统医学中使用的Scoparia dulcis始终如一的强抗氧化特性。 （c）2004 Wiley Periodicals，Inc. J Biochem Mol Toxicol 18：261-272，2004在线发布于Wiley InterScience（www.interscience.wiley.com）。 DOI 10.1002 / jbt.20035。

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