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首页> 外文期刊>Journal of biochemical and molecular toxicology >Methoxychlor-induced alteration in the levels of HSP70 and clusterin is accompanied with oxidative stress in adult rat testis.
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Methoxychlor-induced alteration in the levels of HSP70 and clusterin is accompanied with oxidative stress in adult rat testis.

机译：甲氧氯诱导的成年大鼠睾丸中HSP70和簇蛋白水平的改变伴随着氧化应激。

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摘要

Methoxychlor, an organochlorine pesticide, has been reported to induce abnormalities in male reproductive tract. However, the insight into the mechanisms of gonadal toxicity induced by methoxychlor is not well known. We investigated whether treatment with methoxychlor would alter the levels of stress proteins, heat shock proteins (HSP), and clusterin (CLU), and oxidative stress-related parameters in the testis of adult male rats. Animals were exposed to a single dose of methoxychlor (50 mg/kg body weight) orally and were terminated at various time points (0, 3, 6, 12, 24, and 72 h) using anesthetic ether. The levels of HSP70, CLU, and the activities of superoxide dismutase (SOD), catalase, and lipid peroxidation levels were evaluated in a 10% testis homogenate. A sequential reduction in the activities of catalase and SOD with concomitant increase in the levels of thiobarbituric acid reactive substance (TBARS) was observed. These changes elicited by methoxychlor were very significant between 6-12 h of posttreatment. Immunoblot analysis of HSP revealed the expression of HSP72, an inducible form of HSP, at certain time points (3-24 h) following exposure to methoxychlor. Similarly, the levels of secretory CLU (sCLU) were also found to be elevated between 3-24 h of treatment. The present data demonstrate methoxychlor-elicited increase in the levels of inducible HSP72 and sCLU, which could be a part of protective mechanism mounted to reduce cellular oxidative damage.
机译：据报道，甲氧基氯是一种有机氯农药，可引起男性生殖道异常。但是，关于甲氧氯诱发的性腺毒性机理的见解还不是很清楚。我们调查了甲氧氯处理是否会改变成年雄性大鼠睾丸中应激蛋白，热休克蛋白（HSP）和簇蛋白（CLU）的水平以及氧化应激相关参数。使动物口服单剂量的甲氧基氯（50 mg / kg体重），并在不同的时间点（0、3、6、12、24和72 h）处将其处死。在10％的睾丸匀浆中评估HSP70，CLU的水平以及超氧化物歧化酶（SOD），过氧化氢酶和脂质过氧化的活性。观察到过氧化氢酶和SOD活性的顺序降低，同时硫代巴比妥酸反应性物质（TBARS）的含量随之增加。在治疗后6-12小时之间，甲氧氯引起的这些变化非常显着。 HSP的免疫印迹分析显示，在暴露于甲氧基氯后的某些时间点（3-24小时），HSP72是一种可诱导形式的HSP的表达。同样，在治疗的3-24小时之间，分泌型CLU（sCLU）的水平也升高了。本数据表明甲氧基氯引起的可诱导的HSP72和sCLU的水平增加，这可能是降低细胞氧化损伤的保护机制的一部分。

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