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首页> 外文期刊>Journal of Bioscience and Bioengineering >Rapid Detection of Substrate-Oxidizing Activity of Hiochi Bacteria Using Benzoquinone-Mediated Amperometric Method
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Rapid Detection of Substrate-Oxidizing Activity of Hiochi Bacteria Using Benzoquinone-Mediated Amperometric Method

机译：苯醌介导的安培法快速检测食盐菌的底物氧化活性

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摘要

The substrate-oxidizing activity of sake spoilage lactic acid bacteria (hiochi bacteria) was measured using a benzoquinone-mixed carbon paste electrode on which the bacteria had been immobilized. The electrode im-mersed in a buffer began to produce an anodic current following the addition of n-glucose or gluconate, reflecting the substrate-oxidizing activity of the immobilized bacteria. The time course of the first derivative of the current (dI/dt) exhibited a maximum, the magnitude of which increased with an increase in the number of hiochi bacterial cells immobilized on the electrode. When heat-treated cells were immobilized, the value at the maximum (dI/dtmax) was lower than that obtained with the electrode on which cells which had not been heat-treated had been immobilized. The decrease in dI/dtmax increased at higher heating temperatures and longer heating time. The relative decrease in dI/dtm,~ dne to heat treatment was smaller than the relative decrease in the number of colony formation due to the same treatment, suggesting that cells injured by heat treatment still retain substrate-oxidizing activity.
机译：使用在其上固定有细菌的苯醌混合碳糊电极，测定清酒变质乳酸菌（盐泡菌）的底物氧化活性。加入正葡萄糖或葡萄糖酸盐后，浸入缓冲液中的电极开始产生阳极电流，反映了固定细菌的底物氧化活性。电流的一阶导数的时间过程（dI / dt）表现出最大值，其大小随固定在电极上的日chi细菌细胞数量的增加而增加。当固定热处理的细胞时，最大值（dI / dtmax）低于固定有未热处理的细胞的电极的最大值。 dI / dtmax的降低在较高的加热温度和较长的加热时间下增加。由于相同的处理，相对于热处理的dI / dtm〜dne的相对减少小于集落形成数目的相对减少，这表明受热处理伤害的细胞仍然保留底物氧化活性。

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