...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Biotechnology >Synthesis, physicochemical and immunological properties of oxidized inulin-L-asparaginase bioconjugate
【24h】

Synthesis, physicochemical and immunological properties of oxidized inulin-L-asparaginase bioconjugate

机译：氧化型菊粉-L-天冬酰胺酶生物共轭物的合成，理化和免疫学性质

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In recent years several bioconjugation protocols have been developed to improve the pharmacokinetic and immunological properties of anti-leukemic enzyme, L-asparaginase. In this study we investigated the effect of conjugation with oxidized inulin on these properties. Inulin (MW about 25kDa) was oxidized with periodate and was conjugated to the enzyme at molar ratios of oxidized inulin to enzyme between 2:1 and 4:1. Modified L-asparaginase synthesized at ratio of 2:1 had activity of 65% of that of native enzyme and was used in subsequent experiments. The apparent K(m) of glycoconjugate (0.1 x 10(-4)M) was about five times lower than the K(m) of native enzyme (0.57 x 10(-4)M). Modified L-asparaginase had longer half life, higher thermostability and more resistance to trypsin digestion and better reusability after repeated freezing and wider optimum pH range than that of intact L-asparaginase. The modification of L-asparaginase with oxidized inulin at molar ratio of 2:1 resulted in decreasing antibody (IgG) titer and immunogenecity after repeated injection of rabbits with respect to the native L-asparaginase. Taken together, the results of this study indicated that L-asparaginase modification by oxidized inulin results in a new stabilized product with improved biochemical properties which may be employed for medical purposes.
机译：近年来，已经开发了几种生物缀合方案以改善抗白血病酶L-天冬酰胺酶的药代动力学和免疫学性质。在这项研究中，我们研究了氧化菊粉共轭对这些性质的影响。用高碘酸盐氧化菊粉（MW约25kDa），并以2：1至4：1的氧化菊粉与酶的摩尔比将其与酶偶联。以2：1的比例合成的修饰的L-天冬酰胺酶的活性为天然酶的65％，可用于随后的实验。糖缀合物的表观K（m）（0.1 x 10（-4）M）比天然酶的K（m）（0.57 x 10（-4）M）低约五倍。与完整的L-天冬酰胺酶相比，改性的L-天冬酰胺酶具有更长的半衰期，更高的热稳定性和对胰蛋白酶消化的抵抗力，并且在反复冷冻后具有更好的可重复使用性和更宽的最佳pH范围。用氧化菊粉以2：1的摩尔比修饰L-天冬酰胺酶后，相对于天然L-天冬酰胺酶重复注射兔子后，抗体（IgG）效价和免疫原性降低。两者合计，这项研究的结果表明，氧化的菊粉对L-天冬酰胺酶的修饰产生了一种新的稳定化产品，其生化特性得到改善，可用于医学目的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号