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Engineered affinity proteins-Generation and applications

机译：工程亲和蛋白-产生与应用

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The use of combinatorial protein engineering to design proteins with novel binding specificities and desired properties has evolved into a powerful technology, resulting in the recent advances in protein library selection strategies and the emerge of a variety of new engineered affinity proteins. The need for different protein library selection methods is due to that each target protein pose different challenges in terms of its availability and inherent properties. At present, alternative engineered affinity proteins are starting to complement and even challenge the classical immunoglobulins in different applications in biotechnology and potentially also for in vivo use as imaging agents or as biotherapeutics. This review article covers the generation and use of affinity proteins generated through combinatorial protein engineering. The most commonly used selection techniques for isolation of desired variants from large protein libraries are described. Different antibody derivatives, as well as a variety of the most validated engineered protein scaffolds, are discussed. In addition, we provide an overview of some of the major present and future applications for these engineered affinity proteins in biotechnology and medicine.
机译：使用组合蛋白工程设计具有新颖结合特异性和所需特性的蛋白已发展成为一项功能强大的技术，从而导致蛋白文库选择策略的最新进展以及各种新型工程化亲和蛋白的出现。对不同蛋白质文库选择方法的需求是由于每种目标蛋白质在其可用性和固有特性方面都面临着不同的挑战。目前，替代的工程化亲和蛋白开始在生物技术的不同应用中补充甚至挑战经典的免疫球蛋白，还可能在体内用作成像剂或生物治疗剂。这篇综述文章涵盖了通过组合蛋白工程产生的亲和蛋白的产生和使用。描述了从大蛋白文库中分离所需变体的最常用选择技术。讨论了不同的抗体衍生物，以及各种最有效的工程蛋白支架。此外，我们概述了这些工程化亲和蛋白在生物技术和医学中的一些主要当前和未来应用。

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