Fluorescent DNA: the development of 7-deazapurine nucleoside triphosphates applicable for sequencing at the single molecule level

Seela F; Feiling E; Gross J; Hillenkamp F; Ramzaeva N; Rosemeyer H

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Fluorescent DNA: the development of 7-deazapurine nucleoside triphosphates applicable for sequencing at the single molecule level

机译：荧光DNA：7-脱氮嘌呤核苷三磷酸的开发，可用于单分子水平的测序

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摘要

7-Deaza-2'-deoxyadenosine and -guanosine phosphoramidite building blocks as well as corresponding 5'-triphosphate derivatives are described carrying in position 7 substituents such as iodo, hexyn-1-yl or 5-aminopentyn-1-yl residues. The phosphoramidites were used to synthesize a series of modified oligodeoxynucleotides. A systematic study of the thermal stabilities of these oligonucleotide duplexes demonstrated that the 7-substituents are well accommodated in the major groove of B-DNA. The 7-(aminoalkyn-1-yl)-7-deazapurine 2'-deoxynucleoside triphosphates were labeled with bulky fluorophores such as Rhodamine Green (R) or tetramethylrhodamine.

机译：描述了在位置7上带有取代基如碘，己炔-1-基或5-氨基戊炔-1-基的7-Deaza-2'-脱氧腺苷和-鸟苷亚磷酰胺结构单元以及相应的5'-三磷酸衍生物。亚磷酰胺用于合成一系列修饰的寡脱氧核苷酸。对这些寡核苷酸双链体的热稳定性的系统研究表明，7位取代基很好地容纳在B-DNA的主沟中。 7-（氨基炔基-1-基）-7-脱氮嘌呤2'-脱氧核苷三磷酸用大分子荧光团标记，如若丹明Green（R）或四甲基若丹明。

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来源
《Journal of Biotechnology》 |2001年第3speca期|共11页
作者
Seela F; Feiling E; Gross J; Hillenkamp F; Ramzaeva N; Rosemeyer H;
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正文语种 eng
中图分类生物工程学（生物技术）;
关键词
Oligodeoxynucleotides; Nucleoside triphosphates; Ion-exchange hplc; Dna; Nucleic-acids; Oligonucleotides; Stability; 7-deaza-2'-deoxyguanosines; 2'-deoxyribofuranosides; Chains; Length;

机译：寡脱氧核苷酸;三磷酸核苷;离子交换hplc;Dna;核酸;寡核苷酸;稳定性;7-脱氮2'-脱氧鸟苷;2'-脱氧核糖呋喃糖苷;链条;长度;

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专利

1. Fluorescent DNA: the development of 7-deazapurine nucleoside triphosphates applicable for sequencing at the single molecule level [J] . Seela F, Feiling E, Gross J, Journal of Biotechnology . 2001,第3Speca期

机译：荧光DNA：7-脱氮嘌呤核苷三磷酸的开发，可用于单分子水平的测序
2. Attomole-level Genomics with Single-molecule Direct DNA, cDNA and RNA Sequencing Technologies [J] . Ozsolak Fatih Current Issues in Molecular Biology . 2016,第Null期

机译：具有单分子直接DNA，cDNA和RNA测序技术的原子级基因组学
3. Attomole-level Genomics with Single-molecule Direct DNA, cDNA and RNA Sequencing Technologies [J] . Fatih Ozsolak Current Issues in Molecular Biology . 2016,第1期

机译：具有单分子直接DNA，cDNA和RNA测序技术的原子级基因组学
4. Flow-based continuous DNA sequencing via single molecule detection of enzymatically cleaved fluorescent nucleotides [C] . Jay A. Schecker, Los Alamos National Lab., Los Alamos, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics . 1995

机译：通过单分子检测酶切的荧光核苷酸，基于流的连续DNA测序
5. Functional organization of the Escherichia coli RecA protein: Alteration of the nucleoside triphosphate catalytic domain, indentification of single-stranded DNA binding site(s), and cleavage of the LexA repressor. [D] . Rehrauer, William Michael. 1996

机译：大肠杆菌RecA蛋白的功能组织：核苷三磷酸催化结构域的改变，单链DNA结合位点的鉴定以及LexA阻遏物的裂解。
6. Rolling circle amplification-driven encoding of different fluorescent molecules for simultaneous detection of multiple DNA repair enzymes at the single-molecule level [O] . Chen-chen Li, Hui-yan Chen, Juan Hu, 2020

机译：不同荧光分子的滚动圈扩增驱动编码用于同时检测单分子水平的多个DNA修复酶
7. Characterization of Fluorescent Base Analogs to Study DNA Base Flipping at the Single Molecule Level [O] . Sosa-Vazquez Xochitl A., Vander-Schuur Matthew, Valencia Liza, 2013

机译：表征荧光碱基类似物以研究单分子水平的DNA碱基翻转
8. Development of Single-Molecule DNA Sequencing Platform Based on Single-Molecule Electrical Conductance. [R] . Huang, G. 2015

机译：基于单分子电导的单分子DNa测序平台的研制。

Fluorescent DNA: the development of 7-deazapurine nucleoside triphosphates applicable for sequencing at the single molecule level

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