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首页> 外文期刊>Journal of Biotechnology >In silico and in vivo studies of truncated forms of flagellin (FliC) of enteroaggregative Escherichia coli fused to FimH from uropathogenic Escherichia coli as a vaccine candidate against urinary tract infections
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In silico and in vivo studies of truncated forms of flagellin (FliC) of enteroaggregative Escherichia coli fused to FimH from uropathogenic Escherichia coli as a vaccine candidate against urinary tract infections

机译：在计算机和体内研究截短形式的肠聚集性大肠杆菌鞭毛蛋白（FliC）与来自尿路致病性大肠杆菌的FimH融合作为候选尿道感染的疫苗

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摘要

The new generation of vaccines against infectious diseases is based on recombinant fusion proteins. Flagellin (FliC) of enteroaggregative Escherichia coli (EAEC) could be considered as a potent adjuvant indesigning new vaccines. However, because of its large size, incorporation of this protein with a vaccineantigen might negatively influence recognition of the vaccine epitopes by the immune system. Designingthe truncated forms of FliC, capable of inducing innate immune response, enhances the immune responsesto the target antigen. We have previously shown that two truncated forms of FliC are able to induceInterleukine8 production in HT-29 epithelial cell line. In this study we designed recombinant vaccineagainst urinary tract infections (UTIs) using truncated forms of FliC and type 1 fimbrial FimH adhesin fromuropathogenic Escherichia coli (UPEC) and studied their in silico interactions with Toll-like receptor 5 (TLR5) via docking protocols. The best fusion protein was subjected to cloning and expression. The ability ofthe recombinant vaccine and the truncated forms in inducing immune responses was investigated. Ourresults showed that truncated forms are capable of inducing Th1 (forms A and B) and Th2 (form A) responses and fusion vaccine induced strong cellular and humoral immune responses. (C) 2014 Elsevier B. V. All rights reserved.
机译：新一代的传染病疫苗是基于重组融合蛋白的。肠聚合性大肠杆菌（EAEC）的鞭毛蛋白（FliC）可以被认为是设计新疫苗的有效佐剂。但是，由于其体积较大，因此将该蛋白与疫苗抗原结合可能会对免疫系统对疫苗表位的识别产生负面影响。设计能够诱导先天免疫应答的截短形式的FliC可增强对靶抗原的免疫应答。先前我们已经表明，FliC的两种截短形式能够诱导HT-29上皮细胞系中Interleukine8的产生。在这项研究中，我们使用截短形式的FliC和来自尿路致病性大肠杆菌（UPEC）的1型纤维FimH粘附素设计了针对尿路感染（UTI）的重组疫苗，并通过对接方案研究了它们与Toll样受体5（TLR5）的计算机相互作用。最好的融合蛋白进行了克隆和表达。研究了重组疫苗和截短形式诱导免疫反应的能力。我们的结果表明，截短的形式能够诱导Th1（A和B形式）和Th2（A形式）应答，融合疫苗诱导强烈的细胞和体液免疫应答。 （C）2014 Elsevier B. V.保留所有权利。

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