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首页> 外文期刊>Journal of Biomechanics >Collagen abundance in mechanically stimulated osteoblast cultures using near infrared microscopy
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Collagen abundance in mechanically stimulated osteoblast cultures using near infrared microscopy

机译：机械刺激的成骨细胞培养物中胶原蛋白的丰度使用近红外显微镜

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摘要

Collagen abundance in osteoblast cell cultures was determined using near infrared microscopy with chemical imaging (NIR-CI) with and without mechanical stimulation of the the cells. MC3T3-E1 mouse osteoblast cells seeded on a polycarbonate substrate were mechanically stimulated using static loads of 13.5. N, 27. N and 40. N applied to the substrates during 2, 4, 6 and 8 days of incubation. Results show that the cells increased their collagen production with 13.5. N and 27. N loads when compared to the control sample with a 27. N load resulting in a noteworthy increase (109%) in collagen production. The 40. N load on the other hand, resulted in an initial decrease in the collagen expression in the extracellular matrix, possibly as a result of cell death or inhibition of the protein secretion process followed by an increase in collagen after cell recovery and proliferation. Qualitative confirmation of these results was performed using confocal microscopy.
机译：使用具有和不具有细胞机械刺激的化学成像（NIR-CI）的近红外显微镜，测定成骨细胞培养物中胶原蛋白的丰度。使用13.5的静态载荷机械刺激接种在聚碳酸酯基底上的MC3T3-E1小鼠成骨细胞。在孵育的2、4、6和8天中，将N，27。N和40. N施加到底物上。结果表明，这些细胞的胶原蛋白产量增加了13.5。 N和27. N的负载量与27. N的对照样品相比，导致胶原蛋白生成显着增加（109％）。另一方面，40 N的负荷导致细胞外基质中胶原蛋白表达的最初下降，可能是细胞死亡或抑制蛋白分泌过程的结果，随后是细胞恢复和增殖后胶原蛋白的增加。使用共聚焦显微镜对这些结果进行定性确认。

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