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首页> 外文期刊>Journal of Biomechanics >Region and strain-dependent diffusivities of glucose and lactate in healthy human cartilage endplate
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Region and strain-dependent diffusivities of glucose and lactate in healthy human cartilage endplate

机译：健康人软骨终板中葡萄糖和乳酸的区域和应变依赖性扩散

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The cartilage endplate (CEP) is implicated as the main pathway of nutrient supply to the healthy human intervertebral disc (IVD). In this study, the diffusivities of nutrient/metabolite solutes in healthy CEP were assessed, and further correlated with tissue biochemical composition and structure. The CEPs from non degenerated human IVD were divided into four regions: central, lateral, anterior, and posterior. The diffusivities of glucose and lactate were measured with a custom diffusion cell apparatus under 0%, 10%, and 20% compressive strains. Biochemical assays were conducted to quantify the water and glycosaminoglycan (GAG) contents. The Safranin-O and Ehrlich's hematoxylin and eosin staining and scanning electron microscopy (SEM) were performed to reveal the tissue structure of the CEP. Average diffusivities of glucose and lactate in healthy CEP were 2.68 +/- 0.93 x 10(-7) cm(2)/s and 4.52 +/- 1.47 x 10(-7) cm(2)/s, respectively. Solute diffusivities were region-dependent (p < 0.0001) with the highest values in the central region, and mechanical strains impeded solute diffusion in the CEP (p < 0.0001). The solute diffusivities were significantly correlated with the tissue porosities (glucose: p < 0.0001, r=0.581; lactate: p < 0.0001, r=0.534). Histological and SEM studies further revealed that the collagen fibers in healthy CEP are more compacted than those in the nucleus pulposus (NP) and annulus fibrosus (AF) and show no clear orientation. Compared to human AF and NP, much smaller solute diffusivities in human CEP suggested that it acts as a gateway for solute diffusion through the disc, maintaining the balance of nutritional environment in healthy human disc under mechanical loading and preventing the progression of disc degeneration. (C) 2016 Elsevier Ltd. All rights reserved.
机译：软骨终板（CEP）被认为是向健康人椎间盘（IVD）提供营养的主要途径。在这项研究中，评估了健康CEP中营养素/代谢物溶质的扩散性，并将其与组织生化成分和结构进一步关联。非变性人IVD的CEP分为四个区域：中央，外侧，前部和后部。在0％，10％和20％的压缩应变下，使用常规的扩散池设备测量葡萄糖和乳酸的扩散率。进行生化分析以定量水和糖胺聚糖（GAG）的含量。进行了番红O和Ehrlich的苏木精和曙红染色及扫描电子显微镜（SEM）的检查，以揭示CEP的组织结构。健康CEP中葡萄糖和乳酸的平均扩散率分别为2.68 +/- 0.93 x 10（-7）cm（2）/ s和4.52 +/- 1.47 x 10（-7）cm（2）/ s。溶质扩散率是区域相关的（p <0.0001），在中心区域具有最高的值，并且机械应变阻碍了CEP中的溶质扩散（p <0.0001）。溶质的扩散率与组织孔隙率显着相关（葡萄糖：p <0.0001，r = 0.581;乳酸：p <0.0001，r = 0.534）。组织学和SEM研究进一步表明，健康的CEP中的胶原纤维比髓核（NP）和纤维环（AF）中的胶原纤维更致密，并且没有明确的方向。与人类AF和NP相比，人类CEP中较小的溶质扩散性表明它可作为溶质通过椎间盘扩散的途径，在机械载荷下维持健康人类椎间盘的营养环境平衡，并防止椎间盘退变的进展。 （C）2016 Elsevier Ltd.保留所有权利。

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