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首页> 外文期刊>Journal of Biomechanics >Expression of matrix metalloproteinase genes in the rat intramembranous bone during postnatal growth and upon mechanical stresses
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Expression of matrix metalloproteinase genes in the rat intramembranous bone during postnatal growth and upon mechanical stresses

机译：出生后和机械应激时大鼠膜内骨中基质金属蛋白酶基因的表达

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摘要

A cranial suture consists of neural-crest derived cells and matrices between mineralized skull bones. Little is known regarding the involvement of matrix metalloprotemases (MMPs) in the degradation of extracellular matrix of cranial sutures. In the postnatal rat model, the posterior frontal suture (PFS) undergoes complete ossification between P12-P22, whereas the sagittal suture (SS) remains patent. The present study utilized reverse transcriptase-polymerase chain reaction (RT-PCR) to explore the expression of MMP-1 and MMP-2 genes in the PFS and SS in P8 and P32 rats, and also to determine whether these MMP genes are modulated by exogenous mechanical forces. RNA was isolated from P8 and P32 normal PFS and SS each by pooling sutural specimens from 14 to 20 rats. RT-PCR analysis and semi-quantitative luminosity demonstrated the expression of MMP-1 and MMP-2 genes in the patent P8 PFS, P8 SS, and P32 SS, but no apparent MMP-2 expression in the physiologically ossified P32 PFS. Exogenous cyclic forces applied to the maxilla at 1000 mN and 4 Hz elicited corresponding cyclic bone strain waveforms with peak strain of 134.14 +/- 38.15 muepsilon (mean +/- S.D.) for the PFS, and 28.35 +/- 10.86 muepsilon for the SS in P32 rats. These cyclic forces delivered for 20 min/d over 2 consecutive days induced the expression of MMP-2 gene in the physiologically fused P32 PFS that was not expressed without mechanical stresses. Taken together, these data suggest potentially important roles of MMP genes in the postnatal development of cranial sutures, and their susceptibility to mechanical stresses. (C) 2004 Elsevier Ltd. All rights reserved.
机译：颅骨缝线由神经-衍生的细胞和矿化的颅骨之间的基质组成。关于基质金属旋转酶（MMPs）参与颅骨缝线的细胞外基质降解的研究鲜为人知。在产后大鼠模型中，后额叶缝合线（PFS）在P12-P22之间完全骨化，而矢状缝合线（SS）仍然是专利。本研究利用逆转录聚合酶链反应（RT-PCR）来研究MMP-1和MMP-2基因在P8和P32大鼠的PFS和SS中的表达，并确定这些MMP基因是否受PMP和SS的调节。外力。通过汇集14至20只大鼠的缝合标本，分别从P8和P32正常PFS和SS中分离RNA。 RT-PCR分析和半定量发光证明了专利P8 PFS，P8 SS和P32 SS中MMP-1和MMP-2基因的表达，但在生理上僵化的P32 PFS中没有明显的MMP-2表达。在1000 mN和4 Hz下施加到上颌骨的外源性循环力会引起相应的循环骨应变波形，其中PFS的峰值应变为134.14 +/- 38.15 muepsilon（平均+/- SD），SS的峰值应变为28.35 +/- 10.86 muepsilon在P32大鼠中。这些连续2天/天传递的20分钟/天的循环力诱导了MMP-2基因在生理融合的P32 PFS中的表达，而P32 PFS在没有机械应力的情况下无法表达。综上所述，这些数据表明MMP基因在颅缝的产后发育及其对机械应力的敏感性方面具有潜在的重要作用。 （C）2004 Elsevier Ltd.保留所有权利。

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