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首页> 外文期刊>Journal of Biomechanics >Assessment of composition and anisotropic elastic properties of secondary osteon lamellae.
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Assessment of composition and anisotropic elastic properties of secondary osteon lamellae.

机译：评估次生骨板的组成和各向异性弹性。

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Measurement of the elastic properties of single osteon lamellae is still one of the most demanding tasks in bone mechanics to be solved. By means of site-matched Raman microspectroscopy, acoustic microscopy and nanoindentation the structure, chemical composition and anisotropic elasticity of individual lamellae in secondary osteons were investigated. Acoustic impedance images (911-MHz) and two-dimensional Raman spectra were acquired in sections of human femoral bone. The samples were prepared with orientations at various observation angles theta relative to the femoral long axis. Nanoindentations provided local estimations of the elastic modulus and landmarks necessary for spatial fusion of the acoustic and spectral Raman images. Phosphate nu(1) (961 cm(-1)) and amide I (1665 cm(-1)) band images representing spatial distributions of mineral and collagen were fused with the acoustic images. Acoustic impedance was correlated with the indentation elastic modulus E(IT) (R(2)=0.61). Both parameters are sensitive to elastic tissue anisotropy. The lowest values were obtained in the direction perpendicular to the femoral long axis. Acoustic images exhibit a characteristic bimodal lamellar pattern of alternating high and low impedance values. Since this undulation was not associated with a variation of the phosphate nu(1)-band intensity in the Raman images, it was attributed to variations of the lamellar orientation. After threshold segmentation and conversion to elastic modulus the orientation and transverse isotropic elastic constants were derived for individual ensembles of apparent thin and thick lamellae. Our results suggest that this model represents the effective anisotropic properties of an asymmetric twisted plywood structure made of transverse isotropic fibrils. This is the first report that proves experimentally the ability of acoustic microscopy to map tissue elasticity in two dimensions with micrometer resolution. The combination with Raman microspectroscopy provides a unique way to study bone and mineral metabolism and the relation with mechanical function at the ultrastructural tissue level.
机译：单骨薄片的弹性性能的测量仍然是要解决的骨力学中最苛刻的任务之一。通过位置匹配拉曼光谱，声学显微镜和纳米压痕，研究了次生骨中单个薄片的结构，化学组成和各向异性弹性。在人股骨的切片中获取了声阻抗图像（911-MHz）和二维拉曼光谱。以相对于股骨长轴的各种观察角θ的取向来制备样品。纳米压痕提供了声学模量和光谱拉曼图像空间融合所需的弹性模量和界标的局部估计。代表矿物质和胶原蛋白空间分布的磷酸盐nu（1）（961 cm（-1））和酰胺I（1665 cm（-1））波段图像与声学图像融合在一起。声阻抗与压痕弹性模量E（IT）（R（2）= 0.61）相关。这两个参数都对弹性组织各向异性敏感。在垂直于股骨长轴的方向上获得最低值。声学图像显示出交替的高阻抗值和低阻抗值的特征性双峰层状图案。由于这种起伏与拉曼图像中磷酸盐nu（1）谱带强度的变化无关，因此应将其归因于层状取向的变化。在阈值分割并转换为弹性模量后，得出了明显的薄层和厚层的单个集合的取向和横向各向同性弹性常数。我们的结果表明，该模型代表了由横向各向同性原纤维制成的不对称扭曲胶合板结构的有效各向异性。这是第一份通过实验证明声学显微镜能够以微米分辨率绘制二维组织弹性图的报告。拉曼光谱技术的结合提供了一种独特的方法来研究骨骼和矿物质的代谢以及在超微结构组织水平上与机械功能的关系。

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