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首页> 外文期刊>Journal of Biomechanics >Atomic force microscopy analysis of normal and photoablated porcine corneas.
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Atomic force microscopy analysis of normal and photoablated porcine corneas.

机译：正常和光烧蚀猪角膜的原子力显微镜分析。

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摘要

We showed the capabilities and accuracy of atomic force microscopy (AFM) techniques for imaging and analyzing the corneal epithelium and the photoablated corneal stroma. Eight normal porcine corneas, half of which were ablated using a scanning-spot excimer laser, were examined. All the corneas were imaged in balanced salt solution after fixation in glutaraldehyde. In the normal untreated corneas we observed the epithelial surface showing the typical polygonal cells and presenting numerous microprojections. The superficial epithelial cells were classified in three types as a result of the anterior-surface roughness measurements. AFM images of the photoablated corneal specimens showed undulations and granule-like features on the ablated stromal surface, specific to 193-nm ArF laser irradiation. Nevertheless, the quantitative analysis confirmed the precision of excimer laser surgery in removing sub-micrometric amounts of tissue. AFM showed to be a high-resolved imaging tool for the scanning of both nativeas well as photoablated corneal specimens. Also, this technique permits precise topographic analysis of the corneal plane, in the nanometric scale, of which smoothness is an important physical characteristic and necessary to achieve an optimal optical quality of the eye.
机译：我们展示了原子力显微镜（AFM）技术对成像和分析角膜上皮和光消融角膜基质的能力和准确性。检查了八只正常猪角膜，其中一半使用扫描点准分子激光消融。所有角膜均在戊二醛中固定后在平衡盐溶液中成像。在正常的未经治疗的角膜中，我们观察到上皮表面显示出典型的多边形细胞并呈现出许多微突起。作为表面粗糙度测量的结果，浅表上皮细胞被分为三种类型。光烧蚀的角膜标本的AFM图像在烧蚀的基质表面上显示出起伏和颗粒状特征，这是对193 nm ArF激光照射所特有的。然而，定量分析证实了准分子激光手术在去除亚微米量的组织中的精度。原子力显微镜显示是一种高分辨率的成像工具，可用于扫描本地生物和光烧蚀的角膜标本。而且，该技术允许在纳米尺度上对角膜平面进行精确的形貌分析，其平滑度是重要的物理特性，并且是实现眼睛的最佳光学质量所必需的。

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