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首页> 外文期刊>Journal of Biomechanics >Raman and mechanical properties correlate at whole bone- and tissue-levels in a genetic mouse model.
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Raman and mechanical properties correlate at whole bone- and tissue-levels in a genetic mouse model.

机译：在遗传小鼠模型中，拉曼和机械特性在整个骨骼和组织水平上相关。

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The fracture resistance of bone arises from the composition, orientation, and distribution of the primary constituents at each hierarchical level of organization. Therefore, to establish the relevance of Raman spectroscopy (RS) in identifying differences between strong or tough bone and weak or brittle bone, we investigated whether Raman-derived properties could explain the variance in biomechanical properties at both the whole bone and the tissue-level, and do so independently of traditional measurements of mineralization. We harvested femurs from wild-type mice and mice lacking matrix metalloproteinase 2 because the mutant mice have a known reduction in mineralization. Next, RS quantified compositional properties directly from the intact diaphysis followed by micro-computed tomography to quantify mineralization density (Ct.TMD). Correlations were then tested for significance between these properties and the biomechanical properties as determined by the three-point bending test on the same femurs. Harvested tibia were embedded in plastic, sectioned transversely, and polished in order to acquire average Raman properties per specimen that were then correlated with average nanoindentation properties per specimen. Dividing the nu(1) phosphate by the proline peak intensity provided the strongest correlation between the mineral-to-collagen ratio and the biomechanical properties (whole bone modulus, strength, and post-yield deflection plus nanoindentation modulus). Moreover, the linear combination of nu(1) phosphate/proline and Ct.TMD provided the best explanation of the variance in strength between the genotypes, and it alone was the best explanatory variable for brittleness. Causal relationships between Raman and fracture resistance need to be investigated, but Raman has the potential to assess fracture risk.
机译：骨骼的抗断裂性来自组织各个层次上主要成分的组成，取向和分布。因此，为了确定拉曼光谱（RS）在确定强硬或坚硬骨骼与弱或脆性骨骼之间的差异的相关性，我们研究了拉曼衍生的特性是否可以解释整个骨骼和组织水平的生物力学特性差异，并且与传统的矿化测量方法无关。我们从野生型小鼠和缺乏基质金属蛋白酶2的小鼠中收集股骨，因为突变型小鼠的矿物质含量降低。接下来，RS直接从完整的骨干骨中定量组成成分，然后进行微计算机断层扫描以定量矿化密度（Ct.TMD）。然后测试相关性在这些属性和生物力学属性之间的显着性，如通过在同一股骨上进行三点弯曲测试所确定的。将收获的胫骨埋入塑料中，横向切片并抛光，以获取每个样品的平均拉曼性能，然后将其与每个样品的平均纳米压痕性能相关联。用nu（1）磷酸除以脯氨酸峰强度可提供矿物质胶原蛋白比与生物力学特性（整个骨模量，强度和屈服后挠度加纳米压痕模量）之间的最强相关性。此外，nu（1）磷酸/脯氨酸和Ct.TMD的线性组合提供了基因型之间强度差异的最佳解释，而它单独是脆性的最佳解释变量。需要研究拉曼与骨折阻力之间的因果关系，但拉曼有潜力评估骨折风险。

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