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首页> 外文期刊>Journal of Biomechanics >Analysis of cell viability in intervertebral disc: Effect of endplate permeability on cell population.
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Analysis of cell viability in intervertebral disc: Effect of endplate permeability on cell population.

机译：椎间盘中细胞活力的分析：终板​​通透性对细胞群体的影响。

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摘要

Responsible for making and maintaining the extracellular matrix, the cells of intervertebral discs are supplied with essential nutrients by diffusion from the blood supply through mainly the cartilaginous endplates (CEPs) and disc tissue. Decrease in transport rate and increase in cellular activity may adversely disturb the intricate supply-demand balance leading ultimately to cell death and disc degeneration. The present numerical study aimed to introduce for the first time cell viability criteria into nonlinear coupled nutrition transport equations thereby evaluating the dynamic nutritional processes governing viable cell population and concentrations of oxygen, glucose and lactic acid in the disc as CEP exchange area dropped from a fully permeable condition to an almost impermeable one. A uniaxial model of an in vitro cell culture analogue of the disc is first employed to examine and validate cell viability criteria. An axisymmetric model of the disc with four distinct regions was subsequently used to investigate the survival of cells at different CEP exchange areas. In agreement with measurements, predictions of the diffusion chamber model demonstrated substantial cell death as essential nutrient concentrations fell to levels too low to support cells. Cells died away from the nutrient supply and at higher cell densities. In the disc model, the nucleus region being farthest away from supply sources was most affected; cell death initiated first as CEP exchange area dropped below approximately 40% and continued exponentially thereafter to depletion as CEP calcified further. In cases with loss of endplate permeability and/or disruptions therein, as well as changes in geometry and fall in diffusivity associated with fluid outflow, the nutrient concentrations could fall to levels inadequate to maintain cellular activity or viability, resulting in cell death and disc degeneration.
机译：负责制造和维护细胞外基质，椎间盘细胞通过主要通过软骨终板（CEP）和椎间盘组织从血液供应中扩散而获得必需营养素。转运速率的降低和细胞活性的增加可能不利地干扰复杂的供需平衡，最终导致细胞死亡和椎间盘退变。本数值研究旨在首次将细胞生存力标准引入非线性耦合的营养运输方程，从而评估当CEP交换面积从完全下降时，控制生存细胞种群以及圆盘中氧气，葡萄糖和乳酸浓度的动态营养过程。渗透性条件几乎变为不可渗透性。首先使用椎间盘的体外细胞培养类似物的单轴模型来检查和验证细胞生存力标准。随后使用具有四个不同区域的椎间盘轴对称模型来研究细胞在不同CEP交换区域的存活情况。与测量结果一致，对扩散室模型的预测表明，由于必需营养素的浓度降至支持细胞的水平太低，导致细胞大量死亡。细胞死于营养供应，细胞密度更高。在圆盘模型中，离供应源最远的原子核区域受到的影响最大。当CEP交换面积下降到大约40％以下时，细胞死亡首先开始，随后随着CEP进一步钙化，指数死亡持续至指数耗尽。如果终板通透性丧失和/或破坏，以及与流体流出相关的几何形状变化和扩散率下降，则营养物浓度可能下降至不足以维持细胞活性或活力的水平，从而导致细胞死亡和椎间盘退变。

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