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首页> 外文期刊>Journal of biomedical materials research, Part A >Repair of large cranial defects by hBMP-2 expressing bone marrow stromal cells: Comparison between alginate and collagen type I systems
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Repair of large cranial defects by hBMP-2 expressing bone marrow stromal cells: Comparison between alginate and collagen type I systems

机译：表达hBMP-2的骨髓基质细胞修复大颅骨缺损：海藻酸盐和I型胶原蛋白系统的比较

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Despite a wide range of available sources for bone repair, significant limitations persist. To bioengineer bone, we have previously transferred adenovirus-mediated human BMP-2 gene into autologous bone marrow stromal cells (MSC). We have successfully repaired large, full thickness, cranial defects using this approach. We report now the effectiveness of various hydrogels as the scaffold for this type of bone regeneration, comparing specifically alginate with Type I collagen. Cultured MSC of miniature swine were infected with BMP-2 or β-gal adenovirus 7 days before implantation. These cells were mixed with alginate, ultrapure alginate, alginate-RGD, or type I collagen to fabricate the MSC/biomaterial constructs. The results of cranial bone regeneration were assessed by gross examination, histology, 3D CT, and biomechanical tests at 6 weeks and 3 months after implantation. We found that the BMP-2 MSC/collagen type I construct, but not the β-gal control, effectively achieved nearly complete repair of the cranial defects. No bone regeneration was observed with the other hydrogels. Biomechanical testing showed that the new bone strength was very close and only slightly inferior to that of normal cranial bone. Controlling for the integration of stem cells and ex vivo gene transfer, the alginate scaffolds has a significant negative impact on the success of the construct. Our study demonstrates better bone regeneration by collagen type I over alginate. This may have therapeutic implications for tissue engineered bone repair.
机译：尽管有大量可用于骨修复的来源，但是仍然存在明显的局限性。对于生物工程骨，我们先前已将腺病毒介导的人BMP-2基因转移到自体骨髓基质细胞（MSC）中。我们已经使用这种方法成功地修复了大而全厚度的颅骨缺损。我们现在报告各种水凝胶作为这种骨再生支架的有效性，将藻酸盐与I型胶原进行专门比较。植入前7天，用BMP-2或β-gal腺病毒感染培养的小型猪MSC。将这些细胞与藻酸盐，超纯藻酸盐，藻酸盐-RGD或I型胶原蛋白混合，以制造MSC /生物材料构建体。植入后6周和3个月，通过肉眼检查，组织学，3D CT和生物力学测试评估颅骨再生的结果。我们发现，BMP-2 MSC /胶原I型构建体（而非β-gal对照）有效地实现了几乎完全的颅骨缺损修复。其他水凝胶未观察到骨再生。生物力学测试表明，新骨强度非常接近，仅次于正常颅骨。控制干细胞的整合和离体基因转移，藻酸盐支架对构建物的成功具有显着的负面影响。我们的研究表明，与藻酸盐相比，I型胶原具有更好的骨骼再生能力。这可能对组织工程骨修复具有治疗意义。

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