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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Experimental repair of segmental bone defects in rabbits by angiopoietin-1 gene transfected MSCs seeded on porous β-TCP scaffolds.
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Experimental repair of segmental bone defects in rabbits by angiopoietin-1 gene transfected MSCs seeded on porous β-TCP scaffolds.

机译：血管生成素-1基因转染的MSCs植入多孔β-TCP支架上的实验修复兔节段性骨缺损。

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摘要

Segmental bone defect repair remains a clinical and experimental challenge in tissue engineering with increasing focus on angiogenesis in the bone substitutes. The objective of this study was to investigate the osteogenic effects of angiopoietin-1 (Ang-1) gene transfected bone marrow-derived mesenchymal stem cells (MSCs) seeded on porous β-TCP scaffolds. This bone substitute (experimental group) and MSCs/β-TCP compounds (control group) were implanted into 15 mm segmental bone defects of the radii of 30 New Zealand white rabbits, with platelet-rich plasma injected at the same time. Bone regeneration and angiogenesis were assessed by Scanning electron microscope (SEM), X-ray, histology, immunohistology, and biomechanical outcome measurements made on the 2nd, 4th, 8th, and 12th week after the operation. In vitro, the amount of proliferation and differentiation of Ang-1 gene transfected MSCs was found to be gross increased than that of the control groups. In vivo, a significantly increased amount of new bone formation accompanied by active capillary vasculature regeneration was observed in the pores of the scaffolds which had been seeded with Ang-1 gene transfected MSCs, as compared with the control groups. The biomechanical test confirmed the failure load of new born bone was close to normal bone. These results suggest that transfer of gene encoding Ang-1 to MSCs increases their osteogenic properties by enhancing capillary regeneration, thus providing a rich blood supply for new bone formation in segmental bone defects.
机译：节段性骨缺损修复在组织工程中仍然是临床和实验上的挑战，并且越来越关注骨替代物中的血管生成。这项研究的目的是研究血管生成素-1（Ang-1）基因转染的骨髓间充质干细胞（MSC）植入多孔β-TCP支架的成骨作用。将这种骨替代物（实验组）和MSCs /β-TCP化合物（对照组）植入30只新西兰白兔半径15 mm的节段性骨缺损中，同时注入富含血小板的血浆。术后第2、4、8和12周通过扫描电子显微镜（SEM），X射线，组织学，免疫组织学和生物力学结果测量来评估骨再生和血管生成。在体外，发现Ang-1基因转染的MSC的增殖和分化量比对照组明显增加。在体内，与对照组相比，在用Ang-1基因转染的MSCs接种的支架的孔中观察到新骨形成的数量显着增加，并伴随着主动毛细血管的再生。生物力学测试证实了新生骨骼的破坏负荷接近正常骨骼。这些结果表明，编码Ang-1的基因向MSC的转移通过增强毛细血管再生而增加了其成骨特性，从而为节段性骨缺损中的新骨形成提供了丰富的血液供应。

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