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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Surface design for in situ capture of endothelial progenitor cells: VEGF-bound surface architecture and behaviors of cultured mononuclear cells
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Surface design for in situ capture of endothelial progenitor cells: VEGF-bound surface architecture and behaviors of cultured mononuclear cells

机译：原位捕获内皮祖细胞的表面设计：VEGF结合的表面结构和培养的单核细胞的行为。

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摘要

In situ capture of endothelial progenitor cells (EPCs) in the arterial bloodstream may allow the creation of a functioning endothelium on the luminal surfaces of implanted cardiovascular devices. Our strategy is to use highly biospecific interaction between the cell-surface marker and surface-bound protein. The target-cell marker defined is vascular endothelial growth factor (VEGF) receptor, which is exclusively expressed on endothelial lineage cells. The candidate surface-bound proteins are VEGF and anti-VEGF receptor (VEGF-R1 and VEGF-R2) antibodies, which were covalent-bound on poly(ethylene-co-vinyl alcohol) bearing a high-surface density of hydroxyl groups. Incubating human mononuclear cells on these substrates affected the histochemical expression of cell-surface markers specific for EPCs and endothelial cells (ECs). The VEGF-bound surface significantly increased the number of cells expressing both VEGF receptors after 1 or 2 weeks of culture, whereas both anti-VEGF receptor antibody-bound substrates did not affect the expression of the surface markers, and cells on these surfaces were eventually died. These results indicate that, among the three candidate molecules, VEGF is best able to capture EPCs and induces their differentiation. Additionally, a pilot study of surface architecture of stents and small-diameter artificial grafts was conducted for an ongoing implantation study in a porcine model.
机译：在动脉血流中原位捕获内皮祖细胞（EPC）可允许在植入的心血管装置的腔表面上创建功能正常的内皮。我们的策略是在细胞表面标志物和表面结合蛋白之间使用高度生物特异性的相互作用。定义的靶细胞标志物是血管内皮生长因子（VEGF）受体，仅在内皮谱系细胞上表达。候选的表面结合蛋白是VEGF和抗VEGF受体（VEGF-R1和VEGF-R2）抗体，它们在带有高表面密度羟基的聚（乙烯-共-乙烯醇）上共价结合。在这些底物上孵育人单核细胞会影响EPC和内皮细胞（EC）特异的细胞表面标志物的组织化学表达。培养1或2周后，与VEGF结合的表面显着增加了表达两种VEGF受体的细胞数量，而与抗VEGF受体抗体结合的两种底物均不影响表面标志物的表达，并且最终在这些表面上的细胞死了这些结果表明，在这三种候选分子中，VEGF最能捕获EPC并诱导其分化。另外，进行了支架和小直径人工移植物表面结构的初步研究，用于正在进行的猪模型植入研究。

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