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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Messenger ribonucleic acid expression of 16 matrix metalloproteinases in bone-implant interface tissues of loose artificial hip joints
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Messenger ribonucleic acid expression of 16 matrix metalloproteinases in bone-implant interface tissues of loose artificial hip joints

机译：信使核糖核酸在松动人工髋关节的骨-植入界面组织中16种基质金属蛋白酶的表达

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摘要

Matrix metalloproteinases (MMPs) have been reported to be the major factors responsible for aseptic loosening of artificial hip joints. So far, messenger ribonucleic acid (mRNA) expression patterns of seven MMPs have been reported, but that of many other MMPs which have been newly discovered or recently considered to be responsible for prosthetic loosening is still unknown. In this study, mRNA expression pattern of 16 different types of MMPs were analyzed to evaluate which MMPs were locally produced and contributed to prosthetic loosening. Synovium-like interface tissues between bone and prosthesis were collected from 18 cases of aseptic loose artificial hip joint at revision surgery. Six cases of normal synovium were used as controls. Total RNA was extracted by single-step acid guanidinium-thiocyanate-phenol-chloroform procedure. mRNA expression of MMPs was analyzed by semiquantitative reverse transcription-polymerase chain reaction. Based on local expression pattern of MMPs at the mRNA level, aseptic loose artificial hip joint was characterized by elevated expression of MMP-1, MMP-9, MMP-l0, MMP-12, and MMP-13; moderate expression of MMP-2, MMP-7, MMP-8, MMP-11, membrane type (MT)1-MMP (MMP-14), MT2-MMP (MMP-15), MT3-MMP (MMP-16), MT4-MMP (MMP-17), and MMP-l9; lower expression of MMP-3; and little significance of MMP-20. The MMPs detected in this study can potentially degrade almost all components of the periprosthetic extracellular matrix. Thus, many MMP type enzymes possibly contribute to prosthetic loosening and osteolysis through pathologic extracellular matrix degradation and connective tissue/bone remodeling around prostheses.
机译：基质金属蛋白酶（MMP）已被报道是造成人工髋关节无菌性松动的主要因素。到目前为止，已经报道了七个MMP的信使核糖核酸（mRNA）表达模式，但是新发现或最近被认为与假体松动有关的许多其他MMP的表达模式仍然未知。在这项研究中，分析了16种不同类型的MMP的mRNA表达模式，以评估哪些MMP是局部产生的并有助于假体松动。在翻修手术中，从18例无菌的松动人工髋关节中收集了骨骼和假体之间的滑膜样界面组织。正常滑膜6例作为对照。通过一步一步酸性胍盐-硫氰酸盐-苯酚-氯仿程序提取总RNA。通过半定量逆转录-聚合酶链反应分析MMPs的mRNA表达。基于MMP在mRNA水平的局部表达模式，无菌性松动人工髋关节的特征在于MMP-1，MMP-9，MMP-10，MMP-12和MMP-13的表达升高。 MMP-2，MMP-7，MMP-8，MMP-11，膜类型（MT）1-MMP（MMP-14），MT2-MMP（MMP-15），MT3-MMP（MMP-16）的中等表达，MT4-MMP（MMP-17）和MMP-19 MMP-3的表达较低;对MMP-20的意义不大。在这项研究中检测到的MMPs可能会降解假体周围细胞外基质的几乎所有成分。因此，许多MMP型酶可能通过病理性细胞外基质降解和假体周围的结缔组织/骨重塑而导致假体松动和骨溶解。

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