...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >In vitro bone formation on a bone-like apatite layer prepared by a biomimetic process on a bioactive glass-ceramic.
【24h】

In vitro bone formation on a bone-like apatite layer prepared by a biomimetic process on a bioactive glass-ceramic.

机译：在生物活性玻璃陶瓷上通过仿生过程制备的骨状磷灰石层上的体外骨形成。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study we have investigated the behavior of fetal rat osteoblasts, cultured up to 23 days, on a bioactive apatite-wollastonite (AW) glass-ceramic and on the same material on which a carbonated apatite layer had been formed by a biomimetic process (AWa). At the last day of culture, the specific activity of alkaline phosphatase activity, as determined biochemically, was about 30% greater on AWa compared with AW disks. After the cell layers had been scraped off, scanning electron microscopic (SEM) observations of the materials' surfaces revealed that mineralized bone nodules remained attached to both surfaces but in larger amounts on AWa. X-ray microanalysis indicated the presence of calcium (Ca) and phosphorus (P) in the bone tissue throughout the AWa surface and Ca, P, and silicon (Si) on the AW surface. The AW/ and AWa/bone interfaces also were analyzed after fracturing of the disks. The interfacial analysis showed firm bone bonding to the AW and AWa surfaces, confirmed by the X-ray microanalytic mappings. These results indicate the importance of surface composition in supporting differentiation of osteogenic cells and the subsequent apposition of bone matrix, which allows a strong bond of the bioactive materials to the bone. Furthermore, prefabrication of a biologic apatite layer by a method that mimics biomineralization could find application to bone-repairing materials. Copyright 2000 John Wiley & Sons, Inc.
机译：在这项研究中，我们研究了在生物活性磷灰石-硅灰石（AW）玻璃陶瓷上以及在通过仿生过程在其上形成碳酸磷灰石层的相同材料上培养长达23天的胎儿大鼠成骨细胞的行为（啊！在培养的最后一天，根据生物化学方法测定，碱性磷酸酶活性的比活性比AW盘高约30％。刮下细胞层后，扫描电子显微镜（SEM）对材料表面的观察表明，矿化的骨结节仍然附着在两个表面上，但在AWa上的附着量更大。 X射线显微分析表明，整个AWa表面的骨组织中均存在钙（Ca）和磷（P），而AW表面上的Ca，P和硅（Si）存在。盘破裂后，还分析了AW /和AWa /骨界面。界面分析表明，骨骼牢固地粘结在AW和AWa表面，这已通过X射线微分析图得到了证实。这些结果表明表面组成在支持成骨细胞分化和随后的骨基质并置中的重要性，这允许生物活性材料与骨骼牢固结合。此外，通过模仿生物矿化的方法预制生物磷灰石层可能会应用于骨修复材料。版权所有2000 John Wiley＆Sons，Inc.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号