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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Macrophage reactivity to different polymers demonstrates particle size- and material-specific reactivity: PEEK-QPTIMA~R particles versus UHMWPE particles in the submicron, micron, and 10 micron size ranges
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Macrophage reactivity to different polymers demonstrates particle size- and material-specific reactivity: PEEK-QPTIMA~R particles versus UHMWPE particles in the submicron, micron, and 10 micron size ranges

机译：巨噬细胞对不同聚合物的反应性显示出特定于颗粒尺寸和材料的反应性：亚微米，微米和10微米尺寸范围内的PEEK-QPTIMA〜R颗粒与UHMWPE颗粒

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Biologic reactivity to orthopedic implant debris is generally the main determinant of long-term clinical performance where released polymeric particles of Ultra-high molecular weight polyethylene (UHMWPE) remain the most prevalent debris generated from metal-on-polymer bearing total joint arthroplasties. Polymeric alternatives to UHMWPE such as pol-yetherether-ketone (PEEK) may have increased wear resistance but the bioreactivity of PEEK-OPTIMA particles on peri-implant inflammation remains largely uncharacterized. We evaluated human monocyte/macrophage responses (THP-1s and primary human) when challenged by PEEK-OPTIMA, UHMWPE, and X-UHMWPE particles of three particle sizes (0.7 um, 2 urn, and 10 um) at a dose of 20 particles-per-cell at 24- and 48-h time points. Macrophage responses were measured using cytotox-icity assays, viability assays, proliferation assays and cytokine analysis (IL-1b, IL-6, IL-8, MCP-1, and TNF-alpha). In general, there were no significant differences between PEEK-OPTIMA, UHMWPE, and X-UHMWPE particles on macrophage viability or proliferation. However, macrophages demonstrated greater cyto-toxicity responses to UHMWPE and X-UHMWPE than to PEEK-OPTIMA at 24 and 48 h, where 0.7 mum-UHMWPE particles produced the highest amount of cytotoxicity. Particles of X-UHMWPE more than PEEK-OPTIMA and UHMWPE induced IL-1P, IL-6, MCP-1, and TNF-alpha at 24 h, p < 0.05 (no significant differences at 48 h). On average, cytokine production was more adversely affected by larger 10 u.m particles than by 0.7 and 2 um sized particles. While limitations of in vitro analysis apply to this study, PEEK-OPTIMA particles were more biocompatible than UHMWPE particles, in that they induced less inflammatory cytokine responses and thus, in part, demonstrates that PEEK-OPTIMA implant debris does not represent an increased inflammatory risk over that of UHMWPE.
机译：骨科植入物碎片的生物反应性通常是长期临床表现的主要决定因素，超高分子量聚乙烯（UHMWPE）的已释放聚合物颗粒仍然是携带金属-聚合物的全关节置换术产生的最普遍的碎片。 UHMWPE的聚合物替代品（例如聚醚醚酮（PEEK））可能具有更高的耐磨性，但PEEK-OPTIMA颗粒对植入物周围炎症的生物反应性仍未明确。当以20个颗粒的剂量受到三种粒径（0.7 um，2 um和10 um）的PEEK-OPTIMA，UHMWPE和X-UHMWPE颗粒挑战时，我们评估了人类单核细胞/巨噬细胞反应（THP-1s和原代人类） -每个单元在24和48小时的时间点。巨噬细胞反应使用细胞毒活性测定，生存力测定，增殖测定和细胞因子分析（IL-1b，IL-6，IL-8，MCP-1和TNF-alpha）进行测量。通常，PEEK-OPTIMA，UHMWPE和X-UHMWPE颗粒之间在巨噬细胞生存力或增殖方面没有显着差异。但是，巨噬细胞在24和48小时时表现出比对PEEK-OPTIMA更高的对UHMWPE和X-UHMWPE的细胞毒性反应，其中0.7毫米的UHMWPE颗粒产生最高的细胞毒性。 X-UHMWPE颗粒比PEEK-OPTIMA和UHMWPE颗粒更多，在24 h时诱导IL-1P，IL-6，MCP-1和TNF-α，p <0.05（48 h无明显差异）。平均而言，较大的10 µm颗粒比0.7和2 um大小的颗粒对细胞因子产生的不利影响更大。尽管这项研究应用了体外分析的局限性，但PEEK-OPTIMA颗粒比UHMWPE颗粒具有更高的生物相容性，因为它们诱导的炎症细胞因子反应较少，因此，部分证明了PEEK-OPTIMA植入物碎片并不代表炎症风险增加超过了UHMWPE。

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