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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Effects of density of anisotropic microstamped silica thin films on guided bone tissue regeneration - In vitro study
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Effects of density of anisotropic microstamped silica thin films on guided bone tissue regeneration - In vitro study

机译：各向异性微压印二氧化硅薄膜的密度对引导骨组织再生的影响-体外研究

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The growing demand for better implant aesthetics has led to increased research on the development of all-ceramic dental implants. The use of microtextured coatings with enhanced properties has been presented as a viable way to improve tissue integrability of all-ceramic implants. The aim of this study was to evaluate the effects of different densities of anisotropic microtextured silica thin films, which served as a model coating, on the behavior of human osteoblast-like cells. The differential responses of human osteoblast-like cells to anisotropic silica microtextures with varying densities, produced via a combination of sol-gel and soft lithography processing, were evaluated in terms of alignment, elongation (using fluorescence microscopy), overall cellular activity, and the expression/activity levels of alkaline phosphatase (ALP). Statistical analysis was conducted using one-way ANOVA/Tukey HSD post hoc test. The thin films were thoroughly characterized via scanning electron microscopy/energy dispersive spectroscopy, Fourier transform infrared, and contact angle measurements. Thin film characterization revealed increased nanoscale roughness and reduced wettability on the micropatterned surfaces. Cell culture experiments indicated that the microtextures induced cell alignment, elongation, and guided colonization on the surface. Cells cultured on denser micropatterns exhibited increased metabolic activity (t = 14-21 days). The early expression/activity levels of ALP released into the medium were found to be significantly higher only on the least dense micropattern. These results suggest the possibility that microstructured silica thin films could be used to guide and enhance peri-implant cell/tissue responses, potentially improving tissue integration for metallic and all-ceramic dental implants.
机译：对更好的种植体美学的不断增长的需求导致了对全陶瓷牙齿种植体开发的更多研究。已经提出使用具有增强性能的微织构涂层作为改善全陶瓷植入物的组织可整合性的可行方法。这项研究的目的是评估不同密度的各向异性微织构二氧化硅薄膜（作为模型涂层）对人成骨样细胞行为的影响。通过对准，伸长率（使用荧光显微镜），总体细胞活性和表面活性，评估了人类成骨样细胞对通过溶胶-凝胶和软光刻工艺的结合产生的密度不同的各向异性二氧化硅微观结构的不同反应。碱性磷酸酶（ALP）的表达/活性水平。使用单向方差分析/ Tukey HSD事后检验进行统计分析。通过扫描电子显微镜/能量色散光谱，傅立叶变换红外和接触角测量来彻底表征薄膜。薄膜表征揭示了微图案表面上纳米级粗糙度的增加和润湿性的降低。细胞培养实验表明，微纹理可诱导细胞排列，延伸和在表面引导定植。在较密的微模式上培养的细胞表现出增加的代谢活性（t = 14-21天）。发现释放到培养基中的ALP的早期表达/活性水平仅在最不稠密的微图案上才显着更高。这些结果表明，微结构二氧化硅薄膜可用于引导和增强植入物周围细胞/组织反应的可能性，从而有可能改善金属和全陶瓷牙植入物的组织整合。

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