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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Tetracycline tethered to titanium inhibits colonization by Gram-negative bacteria
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Tetracycline tethered to titanium inhibits colonization by Gram-negative bacteria

机译：拴在钛上的四环素抑制革兰氏阴性细菌的定殖

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摘要

As pen-prosthetic infection is one of the most devastating complications associated with implant placement, we have reasoned that such infection can be largely subverted by development of antibacterial implants. Our previous work demonstrated that covalent coupling of vancomycin to titanium alloy prevented colonization by the Grampositive pathogens, Staphylococcus aureus and Staphylococcus epidermidis. Some orthopedic devices, including permanent prosthesis anchors, and most dental implants are transcutaneous or transmucosal and can be prone to colonization by Gram-negative pathogens. We report here the successful covalent coupling of the broad-spectrum antibiotic, tetracycline (TET), to titanium surfaces (Ti-TET) to retard Gram-negative colonization. Synthetic progress was followed by changes in water contact angle, while the presence of TET was confirmed by immunofluorescence. Ti-TET actively prevented colonization in the presence of bathing Escherichia cob', both by fluorescence microscopy and direct counting. Finally, the Ti-TET surface supported osteoblastic cell adhesion and proliferation over a 72-h period. Thus, this new surface offers a powerful means to protect transcutaneous implants from adhesion of Gram-negative pathogens, decreasing the need for replacement of this hardware. (C) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1381-1389,2015.
机译：由于笔形假体感染是与植入物植入相关的最严重的并发症之一，因此我们认为，这种感染在很大程度上可以通过开发抗菌植入物而得到解决。我们以前的工作表明，万古霉素与钛合金的共价偶联可防止革兰氏阳性病原体，金黄色葡萄球菌和表皮葡萄球菌定植。一些骨科器械，包括永久性假体锚固件，以及大多数牙科植入物都是经皮或透粘膜的，容易被革兰氏阴性病原体定植。我们在这里报告了广谱抗生素四环素（TET）与钛表面（Ti-TET）的成功共价偶联，以阻止革兰氏阴性定植。合成进展之后是水接触角的变化，而免疫荧光证实了TET的存在。通过荧光显微镜和直接计数，Ti-TET可以在沐浴大肠杆菌时有效地阻止定居。最后，Ti-TET表面在72小时内支持成骨细胞粘附和增殖。因此，这种新表面为保护经皮植入物免受革兰氏阴性病原体的粘附提供了有力手段，从而减少了更换该硬件的需求。 （C）2014 Wiley Periodicals，Inc.J Biomed Mater Res Part B：Appl Biomater，103B：1381-1389,2015。

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