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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Osteoinductive-nanoscaled silk/HA composite scaffolds for bone tissue engineering application
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Osteoinductive-nanoscaled silk/HA composite scaffolds for bone tissue engineering application

机译：骨诱导纳米工程化的骨诱导纳米丝/ HA复合支架

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Osteoinductive silk/hydroxyapatite (HA) composite scaffolds for bone regeneration were prepared by combining silk with HA/silk core shell nanoparticles. The HA/silk nanoparticles were directly dispersed in silk solution to form uniform silk/HA blend and then composite scaffolds after a freeze-drying process. The HA/silk nanoparticles uniformly distributed in silk scaffolds at nanometer scale at varying HA content up to 40%, and substantially improved the compressive strength of the scaffolds produced. Rat bone mesenchymal stem cells (rBMSCs) were cultured in these scaffolds and cell proliferation was analyzed by confocal microscopy and DNA assay. Gene expression and biochemical assays were employed to study the influence of increasing HA/silk nanoparticles on in vitro osteogenic differentiation of rBMSCs. Increasing HA/silk nanoparticles inside silk scaffolds improved the growth and osteogenic capability of rBMSCs the absence of osteogenic growth factors, and also significantly increased the calcium and collagen I deposition. In addition, compared to silk/HA composite scaffolds containing HA aggregates, the scaffolds loaded with HA/silk nanoparticles showed remarkably higher stiffness and better osteogenic property at same HA content, implying a preferable microenvironment for rBMSCs. These results suggest that the osteogenic property as well as mechanical property of silk/HA scaffolds could be further improved through fabricating their structure and topography at nanometer scale, providing more suitable systems for bone regeneration. (C) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1402-1414,2015.
机译：通过将蚕丝与HA /蚕丝核壳纳米粒子结合，制备用于骨再生的骨诱导性蚕丝/羟基磷灰石（HA）复合支架。将HA /蚕丝纳米颗粒直接分散在蚕丝溶液中，形成均匀的蚕丝/ HA共混物，然后在冷冻干燥过程中形成复合支架。 HA /丝纳米颗粒以高达40％的不同HA含量均匀分布在纳米级的丝质支架中，并显着提高了所生产支架的抗压强度。在这些支架中培养大鼠骨髓间充质干细胞（rBMSC），并通过共聚焦显微镜和DNA测定分析细胞增殖。基因表达和生化分析被用来研究增加的HA /丝纳米颗粒对rBMSCs体外成骨分化的影响。在不存在成骨生长因子的情况下，增加丝支架中的HA /丝纳米颗粒可改善rBMSC的生长和成骨能力，并且还显着增加钙和I型胶原的沉积。另外，与包含HA聚集体的丝/ HA复合支架相比，负载有HA /丝纳米颗粒的支架在相同的HA含量下显示出显着更高的刚度和更好的成骨特性，这意味着rBMSC具有优选的微环境。这些结果表明，通过在纳米尺度上制备其结构和形貌，可以进一步改善丝绸/ HA支架的成骨性以及机械性能，从而为骨再生提供更合适的系统。 （C）2014 Wiley Periodicals，Inc.J Biomed Mater Res B部分：Appl Biomater，103B：1402-1414，2015。

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