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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Intermolecular force mapping of platelet surfaces on collagen substrata.
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Intermolecular force mapping of platelet surfaces on collagen substrata.

机译：胶原基质上血小板表面的分子间力图。

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摘要

The interactions between plasma proteins and platelets are responsible for surface adsorption and activation of platelets, which leads to initiation of platelet-mediated thrombotic events at biomaterial surfaces. We are seeking to gain a fundamental understanding of these interactions. The atomic force microscope (AFM) has been used to create force maps across platelets adsorbed onto collagen substrata using peptide-modified probes. Combining the imaging and force-measuring capabilities of AFM, the force-mapping mode has been used to measure interactions of peptide-modified AFM probes with the surface. Observed differences in the force of adhesion are clearly evident in the platelet samples fixed in air, proving the ability of the AFM system to map adhesion. When this system is changed to a fluid environment we are no longer able to see such evident adhesion because of the membrane flexibility; instead the deformability of the surface is mapped. The specific interaction between the peptide sequence RGD and platelets was measured in a non-mapping mode of the AFM. Although this does not provide a force map, we can see significant differences between the forces measured on the substrate and those measured with a control hexapeptide. Copyright 1999 John Wiley & Sons, Inc.
机译：血浆蛋白与血小板之间的相互作用负责表面的吸附和血小板的活化，这导致在生物材料表面引发血小板介导的血栓形成事件。我们正在寻求对这些相互作用的基本了解。原子力显微镜（AFM）已用于使用肽修饰探针跨吸附在胶原基质上的血小板创建力图。结合AFM的成像和测力功能，力映射模式已用于测量肽修饰的AFM探针与表面的相互作用。在固定在空气中的血小板样品中，观察到的粘附力差异很明显，证明了AFM系统具有绘制粘附力的能力。当该系统更改为流体环境时，由于膜的柔韧性，我们再也看不到这种明显的附着力。而是映射了表面的可变形性。肽序列RGD和血小板之间的特异性相互作用是在AFM的非映射模式下测量的。尽管这没有提供力图，但我们可以看到在底物上测得的力与对照六肽所测得的力之间存在显着差异。版权所有1999 John Wiley＆Sons，Inc.

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