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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Dysregulation of monocytic cytokine secretion by endodontic sealers.
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Dysregulation of monocytic cytokine secretion by endodontic sealers.

机译：牙髓封闭剂对单核细胞因子分泌的调节异常。

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Recent studies have reported that sealers may alter the secretion of specific cytokines from THP1 monocytic cells in vitro. In this study, a cytokine array was used to determine if endodontic sealers changed secretion of 42 cytokines. White mineral trioxide aggregate (WMTA), MTA preparation (CS), AH-Plus (AHP), and Pulp Canal Sealer (PCS) were mixed, allowed to set for 72 h, then "aged" in buffered-saline for 12 weeks. Aged specimens were placed in direct contact with THP1 for 72 h and their cytotoxicity (MTT assay) was assessed. Materials that were not severely toxic were then exposed to THP1 with or without lipolysaccharide (LPS), and the culture medium was assayed for cytokine secretion. Secretion of cytokines was quantified using infrared scanning (Odyssey((R))); replicate pairs were averaged. PCS severely suppressed MTT activity and was not assessed for its influence on cytokine secretion. WMTA, CS, and AHP induced a broad-based increase in cytokine secretion (>20% vs. Teflon controls), but AHP induced the greatest increase (>100% in 17 of 42 cytokines). The effects of the sealers on LPS-activated THP1 were biphasic, with some increases and decreases cytokine secretion of >20%, but few larger effects. This work shows endodontic sealers may alter the secretion of a broad cross section of cytokines from monocytic cells.
机译：最近的研究报道，封闭剂可能在体外改变THP1单核细胞分泌特定细胞因子的能力。在这项研究中，使用细胞因子阵列来确定牙髓封闭剂是否改变了42种细胞因子的分泌。将白色三氧化二矿骨料（WMTA），MTA制剂（CS），AH-Plus（AHP）和纸浆管封闭剂（PCS）混合，放置72小时，然后在缓冲盐水中“老化” 12周。将老化的标本直接与THP1接触72小时，并评估其细胞毒性（MTT分析）。然后将毒性不严重的材料暴露于有或没有脂多糖（LPS）的THP1中，并分析培养基中细胞因子的分泌。细胞因子的分泌用红外扫描（Odyssey（R））定量。平均重复对。 PCS严重抑制了MTT的活性，尚未评估其对细胞因子分泌的影响。 WMTA，CS和AHP诱导了广泛的细胞因子分泌增加（相对于Teflon对照，> 20％），但是AHP诱导了最大的增加（在42种细胞因子中，有17％> 100％）。封闭剂对LPS激活的THP1的影响是双相的，细胞因子分泌增加和减少的比例> 20％，但影响较小。这项工作表明，牙髓封闭剂可能会改变单核细胞中广泛的细胞因子分泌。

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