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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Matrigel coatings for Parylene sheath neural probes
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Matrigel coatings for Parylene sheath neural probes

机译：用于聚对二甲苯鞘神经探针的Matrigel涂层

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The biologically derived hydrogel Matrigel (MG) was used to coat a Parylene-based sheath intracortical electrode to act as a mechanical and biological buffer as well as a matrix for delivering bioactive molecules to modulate the cellular response and improve recording quality. MG was loaded with dexamethasone to reduce the immune response together with nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) to maintain neuronal density and encourage neuronal ingrowth toward electrodes within the sheath. Coating the Parylene sheath electrode with the loaded MG significantly improved the signal-to-noise ratio for neural events recorded from the motor cortex in rat for more than 3 months. Electron microscopy showed even coverage of both the Parylene substrate and the platinum recording electrodes. Electrochemical impedance spectroscopy (EIS) of coated electrodes in 1x phosphate-buffered saline demonstrated low impedance required for recording neural signals. This result was confirmed by in vivo EIS data, showing significantly decreased impedance during the first week of recording. Dexamethasone, NGF, and BDNF loaded into MG were released within 1 day in 1x phosphate-buffered saline. Although previous studies showed that MG loaded with either the immunosuppressant or the neurotrophic factor cocktail provided modest improvement in recording quality in a 1-month in vivo study, the combination of these bioactive molecules did not improve the signal quality over coating probes with only MG in a 3-month in vivo study. The MG coating may further improve recording quality by optimizing the in vivo release profile for the bioactive molecules. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 357-368, 2016.
机译：生物衍生的水凝胶基质胶（MG）用于涂覆基于聚对二甲苯的鞘内皮层电极，以充当机械和生物缓冲液，以及用于递送生物活性分子以调节细胞反应并改善记录质量的基质。 MG负载了地塞米松，以降低免疫反应，并与神经生长因子（NGF）和脑源性神经营养因子（BDNF）保持神经元密度并促进神经元向鞘内电极的向内生长。用负载的MG涂覆聚对二甲苯鞘电极，可显着改善大鼠运动皮层记录的神经事件的信噪比3个月以上。电子显微镜显示聚对二甲苯基体和铂记录电极均被均匀覆盖。 1x磷酸盐缓冲盐水中的带涂层电极的电化学阻抗谱（EIS）表明，记录神经信号所需的阻抗较低。体内EIS数据证实了这一结果，显示在记录的第一周内阻抗显着降低。装入MG中的地塞米松，NGF和BDNF在1x磷酸盐缓冲液中在1天之内释放。尽管先前的研究表明，在1个月的体内研究中，载有免疫抑制剂或神经营养因子混合物的MG在记录质量方面有适度的改善，但与仅使用MG的包被探针相比，这些生物活性分子的结合并没有改善信号质量。为期3个月的体内研究。 MG涂层可以通过优化生物活性分子的体内释放曲线来进一步改善记录质量。 （c）2015 Wiley Periodicals，Inc. J Biomed Mater Res B部分：Appl Biomater，104B：357-368，2016。

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