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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Osteoclastic responses to various calcium phosphates in cell cultures.
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Osteoclastic responses to various calcium phosphates in cell cultures.

机译：细胞培养物中对各种磷酸钙的破骨反应。

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Disks made of hydroxyapatite, beta-tricalcium phosphate, carbonate apatite, tetracalcium phosphate, alpha-tricalcium phosphate, dicalcium phosphate dihydrate, and octacalcium phosphate were incubated in osteoclastic cell cultures for 2 days. The first five salts were sintered and the last two were compressed before incubation. Osteoclasts resorbed only the sintered carbonate apatite disks. However, osteoclasts were able to resorb octacalcium phosphate disks that were preincubated for 1 day in medium without cells, indicating that surface conditioning was important for osteoclastic resorption of this calcium phosphate. Although resorption did not occur, medium calcium and phosphorus changed to an appreciable extent after a 2-day incubation of beta-tricalcium phosphate, tetracalcium phosphate, alpha-tricalcium phosphate, and dicalcium phosphate dihydrate. These changes in the medium calcium and phosphate concentrations could explain why osteoclasts appeared to have lost their activity on these calcium phosphate disks and were not capable of resorbing them. With hydroxyapatite disks no changes were observed in the medium calcium and phosphorus before and after incubation. Moreover, the osteoclasts appeared to be essentially the same as with the sintered carbonate apatite disks and with bone slices used as a control. Nevertheless, no pits or lacunae were observed on the hydroxyapatite disks, indicating that sintered carbonate apatite should be superior to sintered hydroxyapatite as a bioresorbable bone substitute. Copyright 1999 John Wiley & Sons, Inc.
机译：将由羟基磷灰石，β-磷酸三钙，碳酸磷灰石，磷酸四钙，α-磷酸三钙，磷酸二钙二水合物和磷酸八钙制成的盘在破骨细胞培养物中孵育2天。将前五种盐进行烧结，然后将后两种盐压缩，然后孵育。破骨细胞仅吸收了烧结的碳酸盐磷灰石圆片。但是，破骨细胞能够在没有细胞的培养基中吸收预孵育八天的磷酸八钙片，这表明表面调节对于破骨细胞吸收该磷酸钙很重要。虽然没有发生吸收，但是在β-磷酸三钙，磷酸四钙，α-磷酸三钙和磷酸二钙二水合物孵育2天后，中等的钙和磷变化到一定程度。钙和磷酸盐的中等浓度的这些变化可以解释为什么破骨细胞似乎失去了在这些磷酸钙盘上的活性而不能再吸收它们的原因。用羟基磷灰石圆​​盘孵育之前和之后均未观察到钙和磷的变化。此外，破骨细胞似乎与烧结碳酸盐磷灰石圆盘和以骨切片作为对照的破骨细胞基本相同。然而，在羟基磷灰石圆​​片上未观察到凹坑或凹坑，表明烧结的碳酸盐磷灰石作为生物可吸收性骨替代物应优于烧结的羟基磷灰石。版权所有1999 John Wiley＆Sons，Inc.

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