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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Effects of medium perfusion on matrix production by bovine chondrocytes in three-dimensional collagen sponges
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Effects of medium perfusion on matrix production by bovine chondrocytes in three-dimensional collagen sponges

机译：培养基灌注对三维胶原海绵中牛软骨细胞产生基质的影响

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摘要

Various culture systems have been used for examining the anabolic and catabolic functions of isolated chondrocytes as well as for tissue engineering purposes. Perfusion or frequent medium change is beneficial for three-dimensional (3D) cultures of many cell types. In this study, bovine articular chondrocytes (bACs) were grown in 3D collagen sponges with or without medium perfusion (0.33 mL/ mm) for up to 15 days. The influence of medium perfusion was evaluated using markers of cartilage matrix accumulation, synthesis, and gene expression. Metachromatic matrix, collagen type II, and hyaluronan accumulated around the cells within the collagen sponges. Sulfated glycosaminoglycans (S-GAGs) that accumulated in the sponge exposed to nonperfused control were 130 percent of that in the perfused sponge at day 7. S-GAG accumulation after 15 days in the nonperfused control was 230 percent more than at day 7 (p <0.01). ~(35)S-sulfate incorporation during the final 18 h of culture in the sponge exposed to nonperfusion was 180 percent greater than that in the perfused sponge (p <0.01). Quantitative analyses show that at day 7, aggrecan and collagen type II gene expression were 350 percent and 240 percent greater, respectively, in the nonperfused culture than in the perfused one. These results indicate that perfused conditions that are beneficial for other cell types inhibit chondrogenesis by articular chondrocytes in 3D culture.
机译：各种培养系统已经用于检查分离的软骨细胞的合成代谢和分解代谢功能以及用于组织工程目的。灌注或频繁更换培养基对于许多细胞类型的三维（3D）培养都是有益的。在这项研究中，牛关节软骨细胞（bAC）在3D胶原海绵中生长，有或没有培养基灌注（0.33 mL / mm），最多可持续15天。使用软骨基质积累，合成和基因表达的标志物评估培养基灌注的影响。变色基质，II型胶原和透明质酸聚集在胶原海绵内的细胞周围。在第7天，暴露于非灌注对照组的海绵中积累的硫酸化糖胺聚糖（S-GAG）占灌注海绵的130％，在非灌注对照组中15天后的S-GAG积累比第7天高230％（p <0.01）。在未灌注的海绵中培养的最后18小时，〜（35）S-硫酸盐的掺入量比灌注海绵中的〜（35）S硫酸盐掺入量大180％（p <0.01）。定量分析表明，在第7天，非灌注培养物中的聚集蛋白聚糖和II型胶原蛋白基因表达分别比灌注培养物中高350％和240％。这些结果表明，对其他细胞类型有益的灌注条件抑制了3D培养中关节软骨细胞的软骨生成。

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