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首页> 外文会议>IMECE2008;ASME international mechanical engineering congress and exposition >EFFECTS OF MECHANICAL VIBRATION ON MATRIX PRODUCTION AND PROLIFERATION OF THREE-DIMENSIONAL CULTURED CHONDROCYTES
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EFFECTS OF MECHANICAL VIBRATION ON MATRIX PRODUCTION AND PROLIFERATION OF THREE-DIMENSIONAL CULTURED CHONDROCYTES

机译：机械振动对三维培养软骨细胞基质产生和增殖的影响

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This paper describes the effects of vibration stimulation on chondrocytes in three-dimensional culture in relation to the production of regenerative cartilage tissue, using collagen artificial skin as a carrier and supplementation with hyaluronic acid (used in the conservative treatment of osteoarthritis), and the mechanism of the adaptive response of chondrocytes to mechanical loading. The experimental condition imitates an environment of articular cartilage in vivo that chondrocytes are completely surrounded by the extracellular matrix and receives mechanical stimulation for the weight-bearing mechanics. Chondrocytes were isolated from articular cartilage of porcine metatarsophalangeal joints. Experiments were performed under four different culture conditions: control condition, in which chondrocytes were cultured with atelocollagen gel and collagen artificial skins, and no vibration (HA-Vib-); HA-Vib+, in which chondrocytes were cultured in atelocollagen gel and collagen artificial skins with vibration treatment for 2 weeks; HA+Vib-, in which chondrocytes were cultured in medium containing 0.1% hyaluronic acid; and HA+Vib+, in which chondrocytes were cultured in medium containing 0.1% hyaluronic acid with vibration treatment for 2 weeks. Histologic analysis was conducted at 14 days of culture. The proliferation of chondrocytes was obtained by counting the number of cells with a hemocytometer after 3, 7, 10, and 14 days of culture. The expression of Sox 9 and β-catenin was detected by western blotting analysis. Sox 9 has been reported of involvement in transcription of type IX collagen that binds cartilage-specific type II collagen fibrils.β-catenin plays an important role of signaling pathways of cell proliferation although the relationship between β-catenin and mechanical vibration stimulation has not been clarified yet. The obtained results are as follows. The mechanical vibration enhanced the thickness of extracellular matrix of chondrocytes in histologic section at 14 days of culture and increased the expression of Sox 9. In addition, the mechanical vibration significantly increased the number of chondrocytes after 10 days of culture and promoted the expression of β-catenin. These results show that mechanical vibration promotes the matrix production and proliferation of chondrocytes and that a part of important signaling pathways in relation to mechanical vibration stimulation and proliferation of chondrocytes has been revealed.
机译：本文描述了振动刺激对三维培养物中软骨细胞与再生软骨组织产生的影响，该过程以胶原蛋白人造皮肤为载体并补充透明质酸（用于骨关节炎的保守治疗）及其机理软骨细胞对机械负荷的适应性反应实验条件模拟了体内关节软骨的环境，即软骨细胞完全被细胞外基质包围，并受到机械刺激，从而产生负重的力学作用。从猪meta趾关节的关节软骨分离软骨细胞。实验是在四种不同的培养条件下进行的：对照条件，其中软骨细胞用端胶原蛋白凝胶和胶原蛋白人造皮肤培养，无振动（HA-Vib-）; HA-Vib +，其中软骨细胞在胶原蛋白凝胶和胶原蛋白人造皮肤中经振动处理培养了2周; HA + Vib-，其中软骨细胞在含有0.1％透明质酸的培养基中培养;和HA + Vib +，其中将软骨细胞在含有0.1％透明质酸的培养基中培养并振动处理2周。在培养的第14天进行组织学分析。培养3、7、10和14天后，用血细胞计数器计数细胞数，从而获得软骨细胞的增殖。 Western blotting检测Sox 9和β-catenin的表达。据报道，Sox 9参与结合软骨特异性II型胶原纤维的IX型胶原的转录。 尽管尚未阐明β-连环蛋白与机械振动刺激之间的关系，但β-连环蛋白在细胞增殖的信号传导途径中起着重要的作用。获得的结果如下。机械振动在培养第14天时会增加组织学切片中软骨细胞的细胞外基质厚度，并增加Sox 9的表达。此外，机械振动会在培养10天后显着增加软骨细胞的数量并促进β的表达。 -catenin。这些结果表明机械振动促进软骨细胞的基质产生和增殖，并且已经揭示了与机械振动刺激和软骨细胞增殖有关的重要信号通路的一部分。

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