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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Extracellular Matrix of Human Amnion Manufactured into Tubes as Conduits for Peripheral Nerve Regeneration
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Extracellular Matrix of Human Amnion Manufactured into Tubes as Conduits for Peripheral Nerve Regeneration

机译：人羊膜的细胞外基质制成管作为外周神经再生的管道。

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The human amnion consists of the epithelial cell layer and underlying connective tissue. After removing the epithelial cells, the resulting acellular connective tissue matrix was manufactured into thin dry sheets called amnion matrix sheets. The sheets were further processed into tubes, amnion matrix tubes (AMTs), of varying diameters, with the walls of varying numbers of amnion matrix sheets with or without a gelatin coating. The AMTs were implanted into rat sciatic nerves. Regenerating nerves extended in bundles through tubes of 1-2 mm in diameter and further elongated into host distal nerves 1-3 weeks after implantation. Morphometrical analysis of the regenerated nerve cable at the middle of each amnion matrix tube 3 weeks after implantation was performed The average numbers of myelinated axons were almost the same (ca. 80-112/10~4 mu m~2) in AMTs of 1-2 mm in diameter, as in the normal sciatic nerve (ca. 95/10~4 mu m~2). No myelinated fibers were found in AMTs composed of multiple thin tubes of 0.2 mm in diameter. The myelinated axons were thinner in implanted tubes than those in the normal sciatic nerve. The rate of occurrences of myelinated axons less than 4 mu m in diameter was significantly higher in the AMTs, whereas axons in the normal sciatic nerve were diverse in distribution, with the highest population at 8-12 mu m in diameter. Reinnervation to the gastrocnemius muscle was demonstrated electrophysiologically 9 months after implantation. It was concluded that the extracellular matrix sheet from the human amnion is an effective conduit material for peripheral nerve regeneration.
机译：人羊膜由上皮细胞层和下面的结缔组织组成。除去上皮细胞后，将所得的脱细胞结缔组织基质制成薄的干燥薄片，称为羊膜基质薄片。将该片进一步加工成直径不同的管，羊膜基质管（AMT），其壁具有不同数量的具有或不具有明胶涂层的羊膜基质片的壁。将AMT植入大鼠坐骨神经中。再生神经成束通过直径1-2 mm的管成束延伸，并在植入后1-3周进一步延伸成宿主远端神经。植入后3周，对羊膜基质管中间的再生神经电缆进行形态计量学分析。AMT为1时，髓鞘轴突的平均数目几乎相同（约80-112 / 10〜4μm〜2）。直径为-2 mm，如正常的坐骨神经（约95/10〜4μm〜2）。在由多个直径为0.2 mm的细管组成的AMT中，未发现有髓纤维。植入管中的髓鞘轴突比正常坐骨神经中的轴突细。直径小于4微米的有髓轴突的发生率在AMT中显着更高，而正常坐骨神经中的轴突分布多样，直径最高的是8-12微米。植入后9个月，通过电生理学证实了腓肠肌的神经支配。结论是，人羊膜的细胞外基质片是用于周围神经再生的有效导管材料。

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