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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >False positive results in cytotoxicity testing due to unexpectedly volatile compounds.
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False positive results in cytotoxicity testing due to unexpectedly volatile compounds.

机译：由于意外挥发的化合物，在细胞毒性测试中出现假阳性结果。

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摘要

We investigated the cytotoxicity of different dental materials according to the study protocol adopted by our lab for the screening of new materials. Experimental parameters used in such testing are addressed mainly in documents EN 30993 "Biological evaluation of medical devices, Part 5: Tests for cytotoxicity: in vitro methods" and "Biological evaluation of medical devices, Part 12: Sample preparation and reference materials." Cells were cultured in microplates and challenged with aqueous extracts of the materials. The assay methods were neutral red- and propidium iodide-uptake assays, both indicative of cell viability and able to provide quantitative data. The observation of contrasting results for one material using the above-mentioned methods raised some concern about the assay system used. With further experimentation, it appeared that a sustained release of volatile substances still present in one extract exerted a toxic effect in neighboring cultures. It is concluded that in the microenvironment of a microplate the distribution of samples cannot be disregarded, as it may be responsible for toxicity cross-contamination. Moreover, the use of more than one single method has to be recommended in cytotoxicity testing, in order to avoid false positive results due to experimental artifacts.
机译：我们根据我们实验室通过的研究方案筛选了新材料，调查了不同牙科材料的细胞毒性。此类测试中使用的实验参数主要在文档EN 30993“医疗器械的生物学评估，第5部分：细胞毒性测试：体外方法”和“医疗器械的生物学评估，第12部分：样品制备和参考材料”中得到了解决。在微孔板中培养细胞，并用该材料的水提取物攻击。测定方法是中性碘和碘化丙锭摄取测定，既表明细胞活力，又能提供定量数据。使用上述方法观察一种材料的对比结果引起了人们对所使用的测定系统的担忧。通过进一步的实验，似乎仍然存在于一种提取物中的挥发性物质的持续释放在邻近的培养物中产生了毒性作用。结论是，在微孔板的微环境中，不能忽略样品的分布，因为它可能导致毒性交叉污染。此外，在细胞毒性测试中必须建议使用一种以上的方法，以避免由于实验伪像而导致的假阳性结果。

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