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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >The role of cell density in the survival of cultured cerebellar granule neurons
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The role of cell density in the survival of cultured cerebellar granule neurons

机译：细胞密度在培养的小脑颗粒神经元存活中的作用

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摘要

The dependence for survival of cerebellar granule neurons on the cell density was examined both experimentally and theoretically. The results of batch experiments revealed that the cell survival index (CST) was inappreciable, if cell density was below a critical level. If cell density exceeded this critical value, CSI increased with the increase in cell density. In addition, CSI was significantly increased by using a conditioned medium from the dense cultures. This suggests that not only cell density promotes survival of neurons, but also an increased concentration of growth factors produced by neurons has a direct effect on the survival of the neurons. A quantitative model describing the distribution of the growth factor at different cell densities was proposed to investigate the role of cell density in the survival of the neurons. We showed the existence of a critical level for cell density, and good agreement in the improvement of CSI was found between the theoretical prediction and the experimental result. Finally, the average concentration of growth factor necessary for cell survival based on our model was in a reasonable range compared to the practice of the addition of neurotrophic factors to the medium of cultured cerebellar granule neurons.
机译：实验和理论上检查了小脑颗粒神经元存活对细胞密度的依赖性。批处理实验的结果表明，如果细胞密度低于临界水平，则细胞存活指数（CST）不明显。如果细胞密度超过该临界值，则CSI随着细胞密度的增加而增加。另外，通过使用来自密集培养物的条件培养基，CSI显着增加。这表明不仅细胞密度促进神经元的存活，而且神经元产生的生长因子浓度的增加也直接影响神经元的存活。提出了描述生长因子在不同细胞密度下分布的定量模型，以研究细胞密度在神经元存活中的作用。我们表明存在细胞密度的临界水平，并且在理论上的预测和实验结果之间，在CSI的改善中找到了很好的一致性。最后，与将神经营养因子添加到培养的小脑颗粒神经元培养基中相比，基于我们的模型，细胞存活所必需的生长因子的平均浓度在合理范围内。

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