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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Assembly and characterization of biofunctional neurotransmitter-immobilized surfaces for interaction with postsynaptic membrane receptors
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Assembly and characterization of biofunctional neurotransmitter-immobilized surfaces for interaction with postsynaptic membrane receptors

机译：组装和表征生物功能神经递质的固定表面与突触后膜受体相互作用。

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Herein, we report progress toward the development of bioactive surfaces based on gamma-aminobutyric acid (GABA), a major neurotransmitter in the nervous system. Whereas immobilization techniques have focused largely on antibodies, enzymes, and receptors, to our knowledge, this is the first report of a prototype neurotransmitter-immobilized surface. Biosurfaces were assembled onto either mica or glass using passive adsorption of avidin and subsequent attachment of a derivatized form of GABA via a biotin avidin affinity bond. Surface characterization of these prepared bimolecular surfaces was determined using atomic force microscopy in tapping mode. The data reveal that passive adsorption of avidin is uniformly dispersed and cluster densities can be controlled through the concentration of the avidin incubation solution. GABA tethered via biotin to these avidin surfaces displayed a unique surface topology; in addition, histograms of surface heights suggest two different types of molecular cluster populations. Functional assays were performed to test the biological activity of the synthesized GABA. Anti-GABA antibody directed to these bimolecular surfaces result in morphological topologies and histograms that indicate antibody-antigen binding. However, nonspecific anti-immunoglobulin G antibodies directed to these surfaces show low binding affinity. Taken together, the data support the idea that the synthesized surfaces are biofunctional.
机译：在此，我们报告了基于γ-氨基丁酸（GABA）（一种神经系统中的主要神经递质）的生物活性表面的开发进展。就我们所知，固定化技术主要集中在抗体，酶和受体上，这是固定神经递质的原型表面的首次报道。使用抗生物素蛋白的被动吸附，然后通过生物素抗生物素蛋白亲和力键连接衍生形式的GABA，将生物表面组装到云母或玻璃上。这些准备的双分子表面的表面表征使用原子力显微镜在攻丝模式下确定。数据显示，亲和素的被动吸附均匀分散，并且可以通过亲和素孵育溶液的浓度来控制簇密度。通过生物素连接到这些抗生物素蛋白表面的GABA表现出独特的表面拓扑结构。此外，表面高度的直方图显示了两种不同类型的分子簇种群。进行功能测定以测试合成的GABA的生物学活性。针对这些双分子表面的抗GABA抗体会产生形态学拓扑结构和直方图，表明抗体与抗原的结合。但是，针对这些表面的非特异性抗免疫球蛋白G抗体显示出较低的结合亲和力。综上所述，数据支持合成表面具有生物功能的想法。

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