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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Paraffin Spheres as Porogen to Fabricate Poly(L-Lactic Acid) Scaffolds with Improved Cytocompatibility for Cartilage Tissue Engineering
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Paraffin Spheres as Porogen to Fabricate Poly(L-Lactic Acid) Scaffolds with Improved Cytocompatibility for Cartilage Tissue Engineering

机译：石蜡球作为致孔剂，可制备具有良好细胞相容性的聚（L-乳酸）支架，用于软骨组织工程

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Three-dimensional poly(L-lactic acid) (PLLA) scaffolds with high porosity and pore size ranging from 150 to 700 mu m were conveniently prepared with paraffin spheres used as porogen. PLLA/1,4-dioxane solution containing a given amount of paraffin spheres was frozen at - 25 deg C to obtain a solidified mixture, followed with freeze drying and subsequent leaching with hexane to remove the 1,4-dioxane and paraffin spheres, respectively. The fabricated PLLA scaffolds were highly porous with evenly distributed and interconnected pores. The microstructures of the PLLA scaffolds as a function of paraffin-sphere size, paraffin-sphere dosage, and PLLA concentration were characterized by confocal laser scanning microscopy (CLSM) and scanning-electronic microscopy (SEM). To improve the cyto-compatibility of the bioinert material, a hybrid PLLA scaffold containing Type I collagen was prepared by pressing the collagen solution into the scaffold under reduced pressure. The amounts of the collagen introduced in the scaffolds were detected by ninhydrin method. The distribution of the collagen in the scaffolds was studied with CLSM. Finally, in vitro cell culture was performed by injecting a chondrocyte suspension into the scaffolds. The results showed that the chondrocytes were more evenly distributed and more spread out in the collagen-modified PLLA scaffolds than in the unmodified ones.
机译：使用石蜡球作为致孔剂可以方便地制备具有高孔隙率和150至700μm孔径的三维聚（L-乳酸）（PLLA）支架。将含有给定量的石蜡球的PLLA / 1,4-二恶烷溶液在-25℃冷冻，以获得固化的混合物，然后冷冻干燥，随后用己烷浸提以分别去除1,4-二恶烷和石蜡球。 。制成的PLLA支架高度多孔，具有均匀分布和相互连接的孔。通过共聚焦激光扫描显微镜（CLSM）和扫描电子显微镜（SEM）表征PLLA支架的微观结构与石蜡球大小，石蜡球剂量和PLLA浓度的关系。为了改善生物惰性材料的细胞相容性，通过在减压下将胶原溶液压入支架中来制备含有I型胶原的杂化PLLA支架。通过茚三酮法检测引入到支架中的胶原蛋白的量。用CLSM研究了支架中胶原蛋白的分布。最后，通过将软骨细胞悬浮液注射到支架中进行体外细胞培养。结果显示，胶原修饰的PLLA支架中的软骨细胞比未修饰的软骨细胞更均匀地分布和分布。

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