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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >A Comparative Staining Technique to Detect Mineral Oil Contaminants from Orthopedic Implants
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A Comparative Staining Technique to Detect Mineral Oil Contaminants from Orthopedic Implants

机译：一种从骨科植入物中检测矿物油污染物的比较染色技术

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摘要

An adverse tissue response was reported in patients implanted with porous coated implants containing a mineral oil contaminant. A comparative staining technique was developed in a rabbit and sheep model to identify the presence of a mineral oil contaminant on porous coated implants by examining the surrounding tissues and end organs. The hypothesis for the study was that mineral oil, a saturated lipid, could be distinguished from unsaturated lipids such as animal fats. Frozen rabbit and sheep tissues were stained with hematoxylin and eosin, oil-red-O, and bromide-silver nitrate. Twenty-eight positive control rabbits were injected with mineral oil and 12 negative control rabbits were injected with saline into the paravertebral muscle and synovial cavity of the knee joint. Comparative analysis of tissue was conducted after 1, 3, 5, and 12 weeks. Thermally cleaned porous coated implants were implanted for 6,12, and 24 weeks into cancellous bone of the distal femur of sheep to allow for a weight-bearing model. The comparative histological analysis of the positive control rabbit tissue allowed for detection of mineral oil in all tissues examined. Histological analysis of sheep tissue and saline-injected rabbit tissue showed no evidence of mineral oil or adverse tissue response.
机译：据报道，植入含有矿物油污染物的多孔涂层植入物的患者出现不良组织反应。在兔子和绵羊模型中开发了一种比较染色技术，通过检查周围的组织和末端器官来鉴定多孔涂层植入物上是否存在矿物油污染物。该研究的假设是，可以将饱和脂质的矿物油与动物脂肪等不饱和脂质区分开。将冷冻的兔和绵羊组织用苏木精和曙红，油红-O和溴化物-硝酸银染色。向二十八只阳性对照组的兔子注射矿物油，向十二只阴性对照组的兔子注射盐水至膝关节的椎旁肌和滑膜腔。在1、3、5和12周后进行组织的比较分析。将经过热清洗的多孔涂层植入物植入绵羊股骨远端的松质骨中6,11和24周，以建立负重模型。阳性对照兔组织的比较组织学分析允许在所有检查的组织中检测到矿物油。绵羊组织和注射生理盐水的兔子组织的组织学分析没有发现矿物油或不良组织反应的迹象。

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