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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >In vitro mineralization of a glass-ceramic of the MgO-3CaO centre dot P_2O_5-SiO_2 system: Wettability studies
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In vitro mineralization of a glass-ceramic of the MgO-3CaO centre dot P_2O_5-SiO_2 system: Wettability studies

机译：MgO-3CaO中心点P_2O_5-SiO_2系统的玻璃陶瓷的体外矿化作用：润湿性研究

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摘要

The calcium phosphate deposition on the surface of a bioactive glass-ceramic of the MgO-3CaO centre dot P_2O_5-SiO_2 system during a 1-week immersion in biological model fluids, was investigated through wettability studies. Two model fluids with chemical composition similar to the human blood plasma were tested: Hanks' balanced salt solution (HBSS) and a simulated body fluid (SBF) with a higher calcium content. The effect of the presence of albumin, both in solution or previously adsorbed to the surface, was assessed. The behavior observed using two SBF solutions prepared with and without TRIS buffer was compared. The surface free energy of the glass-ceramic samples was determined, before and after immersion, and dynamic contact angle analysis was performed using the biological model fluids as scanning liquids. Scanning electron microscope observations and x-ray photoelectron spectroscopy (XPS) analysis were performed to complement the wettability studies. The experimental results led to the conclusion that the precipitation of a calcium phosphate film in HBSS occurred mainly when the immersion solution was renewed daily or in the presence of adsorbed albumin. In SBF, the addition of TRIS buffer seemed to inhibit the formation of the calcium phosphate film.
机译：通过润湿性研究，研究了MgO-3CaO中心点P_2O_5-SiO_2系统的生物活性玻璃陶瓷表面在浸泡1周后的磷酸钙沉积情况。测试了两种化学成分类似于人体血浆的模型液体：汉克斯的平衡盐溶液（HBSS）和钙含量较高的模拟体液（SBF）。评估溶液中或先前吸附至表面的白蛋白的存在的影响。比较了使用有和没有TRIS缓冲液制备的两种SBF溶液观察到的行为。在浸没之前和之后确定玻璃陶瓷样品的表面自由能，并使用生物模型流体作为扫描液进行动态接触角分析。进行了扫描电子显微镜观察和X射线光电子能谱（XPS）分析，以补充润湿性研究。实验结果得出的结论是，HBSS中磷酸钙膜的沉淀主要发生在每天更换浸液或存在吸附白蛋白的情况下。在SBF中，TRIS缓冲液的加入似乎抑制了磷酸钙膜的形成。

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