...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Experimental studies on bone induction using low-molecular-weight poly (DL-lactide-co-glycolide) as a carrier for recombinant human bone morphogenetic protein-2
【24h】

Experimental studies on bone induction using low-molecular-weight poly (DL-lactide-co-glycolide) as a carrier for recombinant human bone morphogenetic protein-2

机译：低分子量聚（DL-丙交酯-共-乙交酯）为载体的重组人骨形态发生蛋白2诱导骨的实验研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An appropriate carrier acting as a slow delivery vehicle for the BMPs is required for maximal clinical effectiveness of these bone-inductive proteins. The purpose of this study was to evaluate a low-molecular-weight PLGA copolymer as a synthetic, biodegradable carrier for rhBMP-2 implantation in viva. Two, 10, or 50 mu g of recombinant human BMP-2 were mixed with 10 mg of a poly (DL-lactide-co-glycolide) (PLGA) 50:50 copolymer and implanted into the calf muscles of Wistar rats. Soft X-ray analysis and histologic examination indicated that new bone formation occurred at all rhBMP-2-implanted sites within 3 weeks after implantation. Correlation of rhBMP-2 concentration with the amount of bone induction was confirmed by specific alkaline phosphatase activity and calcium content assay. In vitro analysis indicated that 78.5 percent of the PLGA copolymer was degraded to smaller molecular weight material after 14 days in PBS solution. It is suggested that rhBMP-2 was released in an active form at the implant site during the degradation of the copolymer, resulting in the induction of new bone formation. Thus this low-molecular-weight PLGA copolymer material represents a promising delivery vehicle for BMPs, and possibly other growth factors, around dental and orthopedic implants.
机译：为了使这些骨诱导蛋白具有最大的临床有效性，需要一种适当的载体作为BMP的慢速递送载体。这项研究的目的是评估低分子量PLGA共聚物，作为可生物降解的合成载体，用于rhBMP-2植入体内。将2、10或50μg重组人BMP-2与10 mg聚（DL-丙交酯-共-乙交酯）（PLGA）50:50共聚物混合，然后植入Wistar大鼠的小腿肌肉中。软X射线分析和组织学检查表明，在植入后3周内，所有rhBMP-2植入部位均出现了新的骨形成。通过特异性碱性磷酸酶活性和钙含量测定证实了rhBMP-2浓度与骨诱导量的相关性。体外分析表明，在PBS溶液中放置14天后，有78.5％的PLGA共聚物降解为分子量较小的材料。建议在共聚物降解期间，rhBMP-2以活性形式在植入部位释放，导致诱导新的骨形成。因此，这种低分子量PLGA共聚物材料代表了BMP和牙科和整形外科植入物周围的BMPs以及其他生长因子的有前途的输送载体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号