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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Dissolution control and cellular responses of calcium phosphate coatings on zirconia porous scaffold
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Dissolution control and cellular responses of calcium phosphate coatings on zirconia porous scaffold

机译：氧化锆多孔支架上磷酸钙涂层的溶解控制和细胞响应

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摘要

Different types of calcium phosphates [hydroxy-apatite (HA), fluorapatite (FA), tricalcium phosphate (TCP), and their composites (HA + FA, HA + TCP)] were coated on a zirconia (ZrO_2) porous scaffold using a powder slurry method. The ZrO_2 porous scaffold was intended for a load-bearing implant, and the apatite layers were coated to improve osteoconductivity. The insertion of an FA intermediate layer between the coating layer and ZrO_2 scaffold effectively suppressed the reaction between the calcium phosphate and ZrO_2 and maintained the coating layer at the initial powder composition. The obtained coating layer, of a thickness of approx 30 mum, was relatively microporous and firmly adherent to the ZrO_2 scaffold. Dissolution tests in physiological solution showed typical differences depending on the coating layers, with the dissolution rate increasing in the order TCP > HA + TCP > HA > HA + FA > FA. This result suggests the functional coating of the calcium phosphates in view of tailoring the solubility. Osteoblast4ike cells, MG63 and HOS, responded similarly in terms of cell growth, morphology, and proliferation rate regardless of the coating types, indicating favorable and comparable cell viability. However, the alkaline phosphatase (ALP) activity of the cells on the pure HA and HA composite coatings (HA + FA and HA + TCP) expressed at higher levels compared to those on pure FA and pure TCP coatings for both MG63 and HOS cells, suggesting a selective cell activity depending on the coating types. All the calcium phosphate-coated-ZrO_2 scaffolds showed higher ALP levels compared to pure ZrO_2 scaffold.
机译：使用粉末将不同类型的磷酸钙[羟基磷灰石（HA），氟磷灰石（FA），磷酸三钙（TCP）及其复合物（HA + FA，HA + TCP）]涂覆在氧化锆（ZrO_2）多孔支架上浆料法。 ZrO_2多孔支架旨在用于承重植入物，并涂覆磷灰石层以改善骨传导性。在涂层和ZrO_2支架之间插入FA中间层有效地抑制了磷酸钙和ZrO_2之间的反应，并使涂层保持初始粉末组成。所获得的厚度约为30μm的涂层是相对微孔的并且牢固地粘附至ZrO 2支架。在生理溶液中的溶出度试验显示出取决于涂层的典型差异，溶出度以TCP> HA + TCP> HA> HA + FA> FA的顺序增加。考虑到调节溶解度，该结果表明磷酸钙的功能涂层。无论包被的类型如何，成骨细胞4ike细胞MG63和HOS在细胞生长，形态和增殖速率方面的反应均相似，表明细胞存活力良好且相当。但是，与纯FA和纯TCP涂层的MG63和HOS细胞相比，纯HA和HA复合涂层（HA + FA和HA + TCP）上的细胞碱性磷酸酶（ALP）的表达水平更高，提示取决于涂层类型的选择性细胞活性。与纯ZrO_2支架相比，所有磷酸钙涂覆的ZrO_2支架均显示出更高的ALP水平。

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