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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Three-Dimensional Topographic Scanning Electron Microscope and Raman Spectroscopic Analyses of the Irradiation Effect on Teeth by Nd:YAG, Er:YAG, and CO_2 Lasers
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Three-Dimensional Topographic Scanning Electron Microscope and Raman Spectroscopic Analyses of the Irradiation Effect on Teeth by Nd:YAG, Er:YAG, and CO_2 Lasers

机译:Nd:YAG,Er:YAG和CO_2激光对牙齿的照射效果的三维形貌扫描电子显微镜和拉曼光谱分析

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摘要

A three-dimensional analyzer installed in a scanning electron microscope was used to evaluate the morphology and surface roughness using noncontact profilometry. Observations were carried out on the enamel and dentin surface irradiated by three different lasers: Nd:YAG (wavelength 1.06 mu m), Er:YAG (2.94 /mm), and CO_2 (10.6 mu m). Spectroscopic analysis was done by Raman spectroscopy for nonirradiated and laser-irradiated surfaces. The lasers were applied perpendicularly to vertically sectioned and polished human extracted caries-free molars. The tooth was sectioned at each cavity for cross-section analysis after laser irradiation. Irradiation by Nd:YAG and CO_2 lasers of the enamel surface showed an opaque white color, different from dentin where the surface turned black. The Er:YAG laser induced no changes in color of the dentin. Numerous cracks associated with thermal stress were observed in the CO_2 laser-irradiated dentin. Noncontact surface profile analysis of Er:YAG laser-irradiated enamel and dentin showed the deepest cavities, and direct cross-sectional observations of them showed similar cavity outlines. The CO_2 laser-irradiated dentin had the least surface roughness. Raman spectroscopic analysis showed that fluorescence from the laser-irradiated tooth was generally greater than from nonirradiated teeth. Bands in dentin attributed to organic collagen matrix were lost after Nd:YAG and CO_2 laser irradiation, and a broad peak due to amorphous carbon appeared. The Er:YAG laser-irradiated dentin showed no sign of a carbon band and had more suitable results for dental ablation. Noncontact surface profile analysis was effective to evaluate the structural change in the tooth in the microarea of study after laser irradiation.
机译:使用安装在扫描电子显微镜中的三维分析仪,通过非接触轮廓测量法评估形态和表面粗糙度。在三种不同的激光:Nd:YAG(波长1.06μm),Er:YAG(2.94 / mm)和CO_2(10.6μm)照射的牙釉质和牙本质表面上进行了观察。通过拉曼光谱法对未辐照和激光辐照的表面进行了光谱分析。将激光垂直应用于垂直切开并抛光的人类提取的无龋磨牙。在激光照射后,将牙齿在每个腔处切开以进行横截面分析。 Nd:YAG和CO_2激光照射的釉质表面显示出不透明的白色,这与牙本质变为黑色的牙本质不同。 Er:YAG激光不会引起牙本质颜色的变化。在CO_2激光照射的牙本质中观察到许多与热应力相关的裂纹。 Er:YAG激光照射的牙釉质和牙本质的非接触式表面轮廓分析显示出最深的孔洞,对它们的直接横截面观察显示出相似的孔洞轮廓。经CO_2激光照射的牙本质的表面粗糙度最小。拉曼光谱分析表明,来自激光照射的牙齿的荧光通常要大于未照射的牙齿的荧光。 Nd:YAG和CO_2激光照射后,牙本质中归因于有机胶原基质的条带消失,并出现了由无定形碳引起的宽峰。 Er:YAG激光照射的牙本质未显示出碳带的迹象,并且具有更合适的牙齿消融结果。非接触表面轮廓分析可有效评估激光照射后研究微区中牙齿的结构变化。

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