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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Bone formation using human adipose tissue-derived stromal cells and a biodegradable scaffold.
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Bone formation using human adipose tissue-derived stromal cells and a biodegradable scaffold.

机译：使用人脂肪组织来源的基质细胞和可生物降解的支架进行骨形成。

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摘要

Human adipose tissue, obtained by liposuction, was processed to obtain a fibroblast-like population of cells or adipose tissue-derived stromal cells (ATSCs). The ATSCs, as well as bone marrow-derived mesenchymal stem cells (BMSCs), have the capacity for renewal and the potential to differentiate into multiple lineages of mesenchymal tissues. These cells are capable of forming bone when implanted ectopically in an appropriate scaffold. The aim of this study was to evaluate a beta-tricalcium phosphate (beta-TCP) as a scaffold and to compare the potential of osteogenic differentiation of ATSCs with BMSCs. Both cell types were loaded into beta-TCP disk and cultured in an osteogenic induction medium. Optimal osteogenic differentiation in ATSCs in vitro, as determined by secretion of osteocalcin, scanning electron microscope, and histology, were obtained in the culturing with the beta-TCP disk. Furthermore, bone formation in vivo was examined by using the ATSC- or BMSC-loaded scaffolds in nude mice. The present results show that ATSCs have a similar ability to differentiate into osteoblasts and to synthesize bone in beta-TCP disk as have BMSCs.
机译：通过吸脂获得的人类脂肪组织经过处理，获得了成纤维细胞样细胞或脂肪组织来源的基质细胞（ATSC）。 ATSC以及源自骨髓的间充质干细胞（BMSC）具有更新的能力，并具有分化为多种间充质组织谱系的潜力。当异位植入适当的支架中时，这些细胞能够形成骨骼。这项研究的目的是评估β-磷酸三钙（β-TCP）作为支架，并比较ATSC与BMSC的成骨分化潜力。将两种细胞类型均加载到β-TCP盘中，并在成骨诱导培养基中培养。通过使用β-TCP盘进行培养，可以得到体外骨干细胞中成骨分化的最佳状态，这是由骨钙素的分泌，扫描电子显微镜和组织学确定的。此外，通过在裸鼠中使用ATSC或BMSC负载的支架检查体内的骨形成。目前的结果表明，ATSC具有与BMSC相似的分化为成骨细胞和在β-TCP盘中合成骨骼的能力。

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