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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Heparin-carrying polystyrene (HCPS)-bound collagen substratum to immobilize heparin-binding growth factors and to enhance cellular growth
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Heparin-carrying polystyrene (HCPS)-bound collagen substratum to immobilize heparin-binding growth factors and to enhance cellular growth

机译：携带肝素的聚苯乙烯（HCPS）结合胶原蛋白基质可固定肝素结合生长因子并增强细胞生长

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Heparin-carrying polystyrene (HCPS), consisting of low molecular weight heparin chains linked to a synthetic polystyrene core, is able to attach to polymeric surfaces. In this study, HCPS has efficiently bound to collagen-coated micro-plates and collagen membranes thereby retaining the binding of heparin-binding growth factors, such as vascular endothelial growth factor (VEGF)_(165) or fibroblast growth factor (FGF)-2. Both human skin fibroblast cells and human umbilical vein endothelial cells have shown a good adherence to both collagen- and HCPS-bound collagen substrata. The growth rate of the fibroblast cells on the HCPS-bound collagen substratum in the presence of low concentrations of FGF-2 is higher than on a collagen surface. The fibroblast cells grow at a significantly higher rate on the HCPS-bound collagen substratum retained with FGF-2. Similarly, the growth rate of the endothelial cells on the HCPS-bound collagen substrata in the presence of low concentrations of either FGF-2 or VEGF_(165) is higher than on collagen. The endothelial cells also grow at a significantly higher rate on the HCPS-bound collagen substratum retained with either FGF-2 or VEGF_(165). These results indicate that HCPS-bound collagen substrata with various bioactive heparin-binding molecules may provide novel biomaterials controlling cellular activities such as growth and differentiation.
机译：携带肝素的聚苯乙烯（HCPS）由连接到合成聚苯乙烯核心的低分子量肝素链组成，能够附着在聚合物表面上。在这项研究中，HCPS已有效地结合到胶原蛋白包被的微孔板和胶原膜上，从而保留了结合肝素的生长因子的结合，例如血管内皮生长因子（VEGF）_（165）或成纤维细胞生长因子（FGF）- 2。人皮肤成纤维细胞和人脐静脉内皮细胞均显示出对胶原蛋白和HCPS结合的胶原蛋白基质的良好粘附。在存在低浓度FGF-2的情况下，成纤维细胞在HCPS结合的胶原基质上的生长速率高于在胶原表面上的生长速率。成纤维细胞在以FGF-2保留的HCPS结合的胶原基质上以明显更高的速率生长。类似地，在存在低浓度的FGF-2或VEGF_（165）的情况下，与HCPS结合的胶原蛋白基质上的内皮细胞生长速率高于胶原蛋白。内皮细胞还在以FGF-2或VEGF_（165）保留的HCPS结合胶原基质上以明显更高的速率生长。这些结果表明，具有各种生物活性肝素结合分子的HCPS结合胶原蛋白基质可能提供控制细胞活性（例如生长和分化）的新型生物材料。

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