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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Extracellular matrix degrading enzymes are active in porcine stentless aortic bioprosthetic heart valves
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Extracellular matrix degrading enzymes are active in porcine stentless aortic bioprosthetic heart valves

机译：细胞外基质降解酶在猪无支架主动脉生物假体心脏瓣膜中具有活性

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Glutaraldehyde-fixed porcine aortic valve tissues are widely used for heart valve replacement surgery in the form of bioprosthetic heart valves (BHVs). The durability of BHVs in the clinical setting is limited by tissue degeneration, mechanical failure, and calcification. BHVs rely on the putative ability of glutaraldehyde to render biologic tissues metabolically inert and fully resistant to enzymatic attack. In the present study, we detected and partially characterized the activity of collagen and elastin-degrading enzymes in unimplanted, glutaraldehyde-fixed porcine aortic cusp and wall tissues and compared enzyme activities with those extracted from fresh tissues. Active enzymes capable of degrading extracellular matrix were found to be present in soluble form as well as immobilized on glutaraldehyde-crosslinked tissue matrix. Total levels of collagenolytic activities were evaluated to approximately 0.25 mu g of degraded collagen/mg of dry tissue/24 h for both glutaraldehyde-fixed wall and cusp tissues. A major finding of this study was the ability of soluble tissue enzymes to partially degrade glutaraldehyde-fixed collagen and particularly large amounts of glutaraldehyde-fixed elastin. These calcium-dependent gelatinases share many biochemical similarities with matrix metalloproteinases. These data strongly indicate that glutaraldehyde-fixed porcine valvular tissues are not metabolically inert and are Pot entirely resistant to enzymatic attack, thereby rendering BHVs vulnerable to biologic degeneration and subsequent chronic failure.
机译：戊二醛固定的猪主动脉瓣组织以生物人工心脏瓣膜（BHV）的形式广泛用于心脏瓣膜置换手术。 BHV在临床环境中的持久性受到组织变性，机械衰竭和钙化的限制。 BHV依靠戊二醛的推定能力使生物组织具有代谢惰性，并完全抵抗酶攻击。在本研究中，我们检测并部分表征了未植入，戊二醛固定的猪主动脉瓣和壁组织中胶原和弹性蛋白降解酶的活性，并将酶活性与从新鲜组织中提取的酶活性进行了比较。发现能够降解细胞外基质的活性酶以可溶形式存在并固定在戊二醛交联的组织基质上。对于戊二醛固定的壁和尖瓣组织，胶原蛋白水解活性的总水平被评估为约0.25μg降解的胶原蛋白/ mg干组织/ 24h。这项研究的主要发现是可溶性组织酶能够部分降解戊二醛固定的胶原蛋白，尤其是大量戊二醛固定的弹性蛋白的能力。这些钙依赖性明胶酶与基质金属蛋白酶具有许多生化相似性。这些数据有力地表明，戊二醛固定的猪瓣膜组织不是代谢惰性的，并且对酶的攻击具有完全的抵抗力，从而使BHV容易遭受生物变性和随后的慢性衰竭。

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