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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Analysis of Immobilized L-Cysteine on Polymers
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Analysis of Immobilized L-Cysteine on Polymers

机译：固定在聚合物上的半胱氨酸的分析

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Recently, we reported that L-cysteine attached to polymeric biomaterials, without prior nitrosation, enhances the hemocompatibility of biomaterials via exploiting endogenous nitric oxide (NO). As part of the polymer optimization process to further enhance platelet inhibition, a kinetic model is being developed to predict the release rate of NO. A key model parameter is the immobilized concentration of L-cysteine. This article demonstrates how several chemilu-minescence-based assays, previously utilized for measuring thiols in solution, were successfully adapted to quantify immobilized L-cysteine. The assays showed that the immobilized L-cysteine on the modified PET sample is within the range of 4.1 to 6.5 nmol/cm~2. An advantage of using the more successful chemiluminescence-based assay is that it can accurately measure molar concentrations of any thiol-containing compound with a detection limit in the pmol range. The major disadvantage is that L-cysteine must first be broken off of the polymer and released into solution prior to measurement, therefore leaving the sample unable to be reused. Other thiol-measuring techniques, such as fluorescence microscopy and X-ray photoelectron spectroscopy (XPS), were used to provide qualitative and semiquantita-tive analysis to substantiate the polymer development.
机译：最近，我们报道了在不事先进行亚硝化的情况下，将L-半胱氨酸附着在聚合物生物材料上的方法，是通过利用内源性一氧化氮（NO）来增强生物材料的血液相容性。作为进一步优化血小板抑制作用的聚合物优化过程的一部分，正在开发动力学模型来预测NO的释放速率。模型的关键参数是L-半胱氨酸的固定浓度。本文证明了以前用于测量溶液中硫醇的几种基于化学发光的测定方法如何成功地用于定量固定化的L-半胱氨酸。分析表明，修饰的PET样品上固定的L-半胱氨酸在4.1至6.5nmol / cm〜2的范围内。使用更成功的基于化学发光的分析的一个优点是，它可以准确地测量任何含硫醇的化合物的摩尔浓度，其检测极限在pmol范围内。主要的缺点是L-半胱氨酸必须先从聚合物中分解出来，然后在测量之前释放到溶液中，因此使样品无法重复使用。其他硫醇测量技术，例如荧光显微镜和X射线光电子能谱（XPS），用于提供定性和半定量分析，以证实聚合物的发展。

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