...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Fibrin-Filled Scaffolds for Bone-Tissue Engineering: An In Vivo Study
【24h】

Fibrin-Filled Scaffolds for Bone-Tissue Engineering: An In Vivo Study

机译：用于骨组织工程的纤维蛋白填充支架：体内研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Recently, fibrin sealants that typically contain supra physiological concentrations of fibrinogen and thrombin have been investigated as matrices to facilitate the delivery of cells within biodegradable scaffolds for tissue engineering applications. It is well known from in vitro experiments that the thrombin concentration present during fibrin polymerization influences the structural properties of fibrin, and these can affect cell invasion. This study was conducted to determine whether the structural properties of fibrin can affect bony "wound healing in vivo. Drill hole defects were created in the distal femurs of 20 rats. Four experimental groups were used: nontreated defects, scaffolds alone, and scaffolds filled with fibrin polymerized with either a low thrombin concentration [fibrin(low T)] or a high thrombin concentration [fibrin(high T)]. The area of bone formed at 2, 5, and 11 days after implantation was determined histomorphometrically. After 5 days, scaffolds filled with fibrin(high T) were infiltrated with less bone than empty scaffolds (p < 0.05), but no statistical difference was found between the empty scaffolds and the scaffolds filled with fibrin(low T). After 11 days, both fibrin-filled scaffolds significantly delayed bony wound healing (p < 0.004). Reducing sodium dodecyl sulfate polyacrylamide gel electro-phoresis analysis of the two fibrin formulations showed no difference in gamma-gamma crosslink formation. This work demonstrates that fibrin sealants in their present state are not ideal for enhancing bone-tissue invasion into scaffolds, and that the structural properties of fibrin matrices may be an important design parameter for maximizing host tissue invasion during wound healing.
机译：近来，已经研究了通常包含超生理浓度的纤维蛋白原和凝血酶的纤维蛋白密封剂作为基质，以促进在组织工程应用的可生物降解支架内递送细胞。从体外实验众所周知，在纤维蛋白聚合期间存在的凝血酶浓度影响纤维蛋白的结构性质，并且这些可以影响细胞侵袭。进行这项研究以确定纤维蛋白的结构性质是否会影响体内“骨”伤口愈合。在20只大鼠的股骨远端产生了钻孔缺损。使用了四个实验组：未经治疗的缺损，仅使用脚手架和填充有脚手架的脚手架低凝血酶浓度[fibrin（low T）]或高凝血酶浓度[fibrin（high T）]聚合的纤维蛋白，用组织形态学方法测定植入后2、5和11天形成的骨面积。 ，充满纤维蛋白（高T）的支架的骨浸润少于空支架（p <0.05），但空支架和充满纤维蛋白（低T）的支架之间无统计学差异。填充的支架显着延迟了骨伤口的愈合（p <0.004）。降低十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析两种纤维蛋白制剂的结果表明，γ-γ交联形式没有差异信息。这项工作表明，目前处于状态的血纤蛋白密封剂对于增强骨组织向支架的浸润不是理想的，并且血纤维蛋白基质的结构特性可能是在伤口愈合期间最大化宿主组织浸润的重要设计参数。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号