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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Responses of Human Keratocytes to Micro- and Nanostructured Substrates
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Responses of Human Keratocytes to Micro- and Nanostructured Substrates

机译：人角质形成细胞对微和纳米结构基质的反应。

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We have previously shown that human corneal epithelial cells respond to synthetic topographic features with dimensions similar to those found in the native human corneal basement membrane. Epithelial cells integrated inputs from substrate topography and soluble factors in the culture medium to generate alignment responses to substrate topographic anisotropies. Human keratocytes are the main cellular components of the stroma, the tissue that underlies the corneal epithelium. Here we report that keratocytes aligned more strongly than epithelial cells along topographic patterns of grooves and ridges. On patterns with pitches of 800 nm and larger approximately 70 percent of keratocytes were aligned along the patterns compared to 35 percent for epithelial cells. On 70 nm-wide ridges on a 400-nm pitch, keratocyte alignment dropped to 45 percent, whereas epithelial cell alignment remained constant. Similarly to epithelial cells, focal adhesions and associated stress fibers in keratocytes were aligned mainly along the substrate topographies, although oblique orientations were also observed. Furthermore, keratocytes cultured on the nanoscale patterns had fewer stress fibers and focal adhesions than cells cultured on microscale patterns or on smooth substrates.
机译：先前我们已经表明，人角膜上皮细胞对合成的地形特征有响应，其尺寸与在天然人角膜基底膜中发现的尺寸相似。上皮细胞整合了培养基中底物形貌和可溶性因子的输入，以产生对底物形貌各向异性的比对响应。人角质细胞是基质（角膜上皮基础组织）的主要细胞成分。在这里我们报告角质细胞排列比沟槽和脊的地形图样上皮细胞更强。在间距为800 nm和更大的图案上，约有70％的角膜细胞沿该图案排列，而上皮细胞为35％。在400 nm间距的70 nm宽脊上，角膜细胞排列率下降到45％，而上皮细胞排列率保持恒定。与上皮细胞相似，尽管也观察到倾斜方向，但角膜细胞中的粘着粘附和相关的应力纤维主要沿基底形貌排列。此外，与在微尺度图案或光滑基质上培养的细胞相比，在纳米尺度图案上培养的角膜细胞具有更少的应力纤维和粘着斑。

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