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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >The internalized CdSe/ZnS quantum dots impair the chondrogenesis of bone marrow mesenchymal stem cells.
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The internalized CdSe/ZnS quantum dots impair the chondrogenesis of bone marrow mesenchymal stem cells.

机译：内在的CdSe / ZnS量子点损害了骨髓间充质干细胞的软骨形成。

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摘要

Mesenchymal stem cells (MSCs) are capable of differentiating into multiple cell lineages and are useful for therapeutic applications. Labeling the MSCs with fluorescent probes is beneficial in tracing the fate of MSCs after implantation. We have introduced the CdSe/ZnS quantum dots (QDs) into the human bone marrow MSCs and examined the effects of QDs on the proliferation and chondrogenesis of the cells. The internalized QDs were found localized in perinuclear regions and remained there after a number of cell passages. The presence of QDs did not affect the proliferation of cells or the size of chondrospheres formed, when subjected to chondrogenesis induction. However, the expression of mRNA and protein of type II collagen and aggrecan in the chondrospheres was significantly inhibited in cells labeled with QDs, suggesting impaired chondrogenesis. Our results that the presence of QDs interferes with the chondrogenic differentiation of MSCs raise concerns in using the QDs as fluorescence tracers for stem cells.
机译：间充质干细胞（MSC）能够分化为多种细胞谱系，可用于治疗应用。用荧光探针标记MSC有助于追踪植入后MSC的命运。我们已经将CdSe / ZnS量子点（QDs）引入人骨髓MSC，并研究了QDs对细胞增殖和软骨形成的影响。发现内在的QD位于核周区域，并在经过许多次细胞传代后仍然存在。当进行软骨形成诱导时，QD的存在不影响细胞的增殖或形成的软骨球的大小。但是，在QD标记的细胞中，软骨球中II型胶原蛋白和聚集蛋白聚糖的mRNA和蛋白的表达被显着抑制，表明软骨形成受损。我们关于QD的存在干扰MSC软骨分化的结果引起了人们对使用QD作为干细胞荧光示踪剂的担忧。

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