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首页> 外文期刊>Journal of biomedical science. >Coordinated expression and immunogenicity of an outer membrane protein from Salmonella enterica serovar Typhi under iron limitation, oxidative stress and anaerobic conditions.
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Coordinated expression and immunogenicity of an outer membrane protein from Salmonella enterica serovar Typhi under iron limitation, oxidative stress and anaerobic conditions.

机译：在铁限制，氧化应激和厌氧条件下，鼠伤寒沙门氏菌伤寒沙门氏菌外膜蛋白的协同表达和免疫原性。

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摘要

Successful pathogens overcome the environmental stresses by the coordinated expression of various genes and eventually proteins. Since, the surface of the microbe is likely to come in contact with the host initially, an attempt was made to identify the outer membrane proteins (OMPs), if any, which may get expressed under more than one environmental conditions simulating the in vivo ones. In the present study, Salmonella enterica serovar Typhi was grown under iron-limited, oxidative stress as well as anaerobic conditions and the OMP profiles were compared. A 69 kDa OMP was found to express with enhanced intensity under the selected stress conditions in comparison to normal conditions. The phenotypic similarity among the proteins was assessed on the basis of their molecular weight, cross reactivity and HPLC. The protein expressed under oxidative stress and anaerobic conditions reacted with the antibodies raised against iron-regulated outer membrane protein (IROMP), indicating the sharing of at least someof the epitopes. A single peak observed after subjecting the pooled 69 kDa protein sample and appearance of a single band on SDS-PAGE thereafter, confirmed the purity and phenotypic similarity of the 69 kDa OMP. Reactivity of pooled 69 kDa protein with 85% of sera from typhoid patients revealed the in vivo expression of this protein. The results of this study indicate the coordination of this phenotype under iron stress, oxidative stress and anaerobic conditions. In view of the expression of the 69 kDa protein under the selected stress conditions and their in vivo immunogenicity, these findings may be relevant for the better understanding of the host-microbe interactions and for the further development of diagnostic and preventive strategies.
机译：成功的病原体通过各种基因和最终蛋白质的协同表达克服了环境压力。由于微生物的表面很可能最初会与宿主接触，因此尝试鉴定外膜蛋白（OMP）（如果有），该蛋白可能会在多个模拟体内条件的环境条件下表达。在本研究中，肠炎沙门氏菌伤寒沙门氏菌在铁受限，氧化应激以及厌氧条件下生长，并比较了OMP谱。与正常条件相比，发现在选定的压力条件下，一个69 kDa的OMP可以增强表达。根据蛋白质的分子量，交叉反应性和HPLC评估蛋白质之间的表型相似性。在氧化应激和厌氧条件下表达的蛋白质与针对铁调节的外膜蛋白质（IROMP）产生的抗体反应，表明至少有一些表位共有。对合并的69 kDa蛋白样品进行处理后观察到一个单峰，此后在SDS-PAGE上出现一条条带，证实了69 kDa OMP的纯度和表型相似性。合并的69 kDa蛋白与伤寒患者血清的85％的反应性表明该蛋白在体内的表达。这项研究的结果表明在铁胁迫，氧化应激和厌氧条件下该表型的协调性。鉴于在选定的应激条件下69 kDa蛋白的表达及其体内免疫原性，这些发现可能与更好地了解宿主-微生物相互作用以及进一步发展诊断和预防策略有关。

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