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首页> 外文期刊>Journal of biomedical nanotechnology >Biocompatibility testing of single-walled carbon nanotubes on murine preosteoblasts: Higher osteoblastic differentiation with BMP-9 than with BMP-2
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Biocompatibility testing of single-walled carbon nanotubes on murine preosteoblasts: Higher osteoblastic differentiation with BMP-9 than with BMP-2

机译：鼠前成骨细胞上单壁碳纳米管的生物相容性测试：BMP-9比BMP-2具有更高的成骨细胞分化能力

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Carbon nanotubes (CNTs) have been used in orthopaedic applications because of their exceptional mechanical properties. However, the influence of CNTs on the behaviour of bone-forming cells and on the ability of these cells to respond to growth factors, such as bone morphogenetic proteins (BMPs), remains poorly known. Therefore, in the present study, single-walled CNTs (SWCNTs) were synthesised using an induction thermal plasma process and purified using a multistep procedure. The impact of these purified SWCNTs on the Smad activation, cell proliferation and differentiation, with or without BMP-2 and BMP-9 (1.92 nM), was also studied using western blot, mitochondrial enzymatic activity, TUNEL, RT-PCR and alkaline phosphatase activity analyses. Pre-treatment of MC3T3-E1 preosteoblasts with SWCNTs accelerated the Smad1/5/8 activation, induced by both BMP-2 and BMP-9, within 15 min. It also slightly affected their proliferation at 48 h without apoptosis. Interestingly, at 72 h, BMP-9 favoured the differentiation of MC3T3-E1 preosteoblasts pretreated with SWCNTs to a larger extent than BMP-2 did. Therefore, the combination of BMP-9 with SWCNTs appears to be a promising avenue for bone applications.
机译：碳纳米管（CNT）由于其卓越的机械性能而已被用于整形外科。但是，碳纳米管对骨形成细胞的行为以及这些细胞对生长因子（例如骨形态发生蛋白（BMP））的反应能力的影响仍然知之甚少。因此，在本研究中，使用感应热等离子体工艺合成单壁CNT（SWCNT），并使用多步程序对其进行纯化。还使用蛋白质印迹，线粒体酶活性，TUNEL，RT-PCR和碱性磷酸酶研究了这些纯化的SWCNT在有或没有BMP-2和BMP-9（1.92 nM）时对Smad活化，细胞增殖和分化的影响。活性分析。用SWCNT预处理MC3T3-E1成骨细胞在15分钟内加速了BMP-2和BMP-9诱导的Smad1 / 5/8活化。它还在48小时没有细胞凋亡的情况下轻微影响了它们的增殖。有趣的是，在72小时时，BMP-9促进了用SWCNTs预处理的MC3T3-E1前成骨细胞的分化，其程度大于BMP-2。因此，BMP-9与SWCNT的结合似乎是骨应用的有前途的途径。

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