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首页> 外文期刊>Journal of biomedical science. >Morphogenesis of the hepatitis B virion and subviral particles in the liver of transgenic mice.
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Morphogenesis of the hepatitis B virion and subviral particles in the liver of transgenic mice.

机译：转基因小鼠肝脏中乙型肝炎病毒粒子和亚病毒颗粒的形态发生。

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Up to date, no ultrastructure showing the HBV budding site has ever been reported. In this study, the liver of transgenic mice expressing a high titer of HBV was processed for cryo-ultrathin section electron microscopy. This approach preserves membrane structures very well and thus allowed us to find HBV (Dane particles) and subviral particles (spherical and filamentous form) present separately inside the lumen of the endoplasmic reticulum (ER). Envelopment of single core particle by the ER was found near the Golgi region and mature Dane particles usually resided singularly in a 70-90 nm vesicle at the end of ER or near to mitochondria. Filamentous particles, either in an array or as a single filament inside various sizes of vesicles, were most frequently; these particles were found adjacent to the Golgi region or bile canaliculi. The formation of 22-nm spherical particles seems to occur inside the ER by a transition from preformed filaments to a structure similar to beads on a string. This study is the first report to demonstrate a serial process by which hepatitis B virion assembly takes place in the ER region and distinguishes two separate routes for the morphogenesis of virions and subviral particles.
机译：迄今为止，还没有报道过显示HBV出芽部位的超微结构。在这项研究中，对表达高滴度HBV的转基因小鼠的肝脏进行了超低温切片电子显微镜处理。这种方法很好地保留了膜的结构，因此使我们能够发现内质网（ER）内腔中分别存在的HBV（戴恩颗粒）和亚病毒颗粒（球形和丝状）。在高尔基体区域附近发现了ER包裹单核颗粒，成熟的Dane颗粒通常在ER末端或接近线粒体的70-90 nm囊泡中单独存在。最常见的是呈丝状的颗粒，呈阵列排列或呈单丝状，位于各种大小的囊泡中。这些颗粒被发现在高尔基体或胆小管附近。 22 nm球形颗粒的形成似乎是在ER内部发生的，这是因为从预形成的细丝过渡到类似于弦上的小珠的结构。这项研究是第一份证明乙型肝炎病毒颗粒在内质网区域发生的系列过程的报告，并区分了病毒颗粒和亚病毒颗粒形态发生的两种不同途径。

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