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首页> 外文期刊>Journal of Biomolecular Structure and Dynamics >Multinuclear NMR Investigation of the NaDNA/Ethidium Bromide Anisotropic System.
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Multinuclear NMR Investigation of the NaDNA/Ethidium Bromide Anisotropic System.

机译:NaDNA /溴化乙锭各向异性系统的多核NMR研究。

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摘要

A combined use of (31)P, (23)Na, (2)H and (17)O NMR spectroscopies and polarized light microscopy has been employed to investigate the effect of the ethidium bromide (EB) binding on the liquid crystalline phase of concentrated double stranded DNA solutions. The optical textures and the (31)P and (23)Na NMR spectra of the DNA anisotropic solutions show that the intercalation of EB induces significant modifications either in the arrangements of the DNA rods and the surrounding ionic atmosphere. On the contrary, no indication of significant changes of the orientational order of the water molecules around DNA emerges from the water (2)H and (17)O NMR spectra.
机译:(31)P,(23)Na,(2)H和(17)O NMR光谱学和偏光显微镜的结合使用已被用于研究溴化乙锭(EB)结合对液晶相的影响。浓缩双链DNA溶液。 DNA各向异性溶液的光学织构以及(31)P和(23)Na NMR光谱表明,EB的插入在DNA棒的排列和周围的离子气氛中均引起显着的修饰。相反,从水的(2)H和(17)O NMR光谱中看不出DNA周围水分子的取向顺序有明显变化的迹象。

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